Characterization of polysaccharide production of Haemophilus influenzae type b and its relationship to bacterial cell growth

Autor(es)

Takagi, Mickie ; Cabrera-Crespo, Joaquin ; Baruque-Ramos, Júlia ; Zangirolami, Teresa Cristina ; Raw, Isaias ; Tanizaki, Martha Massako ^[6]

• Tipo de documento: Artigo Científico
• Fonte: Applied Biochemistry and Biotechnology; v. 110, n. 2, p. 91-100, Aug. 2003.
• Área do conhecimento: Ciências Biológicas - Microbiologia
• Assunto(s): Bactérias Haemophilus influenzae tipo B Polissacarídeos Biopolímeros

Resumo

Haemophilus influenzae type b (Hib) causes invasive infections in infants and young children. Vaccines consisting of Hib capsular polysaccharide (polymer of ribosylribitol phosphate [PRP]) conjugated to a protein are effective in the prevention of such infections. The production of capsular polysaccharide type b was studied in three cultivation conditions: single, glucose pulse, and repeated batch. Specific polysaccharide production (Y p/x ) was calculated for all experiments, showing the following values: 67 (single-batch cultivation), 71 (glucose pulse), 75 (repeated-batch cultivation, first batch), and 87 mg of PRP/g of dry cell weight (DCW) (repeated-batch cultivation, second batch). Biomass concentration reached ~1.8 g of DCW/L, while polysaccharide concentration was about ~132 mg/L in the three fermentation runs. Polysaccharide synthesis is associated with cell growth in all studied conditions as established by Kono's analysis and Luedeking-Piret's model. (AU)

• Processo FAPESP: 00/08464-6 - Vacinas conjugadas anti-meningite: da bancada para escala piloto
• Beneficiário: Martha Massako Tanizaki
• Modalidade de apoio: Auxílio à Pesquisa - Regular