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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of polysaccharide production of Haemophilus influenzae type b and its relationship to bacterial cell growth

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Author(s):
Takagi, Mickie ; Cabrera-Crespo, Joaquin ; Baruque-Ramos, Júlia ; Zangirolami, Teresa Cristina ; Raw, Isaias ; Tanizaki, Martha Massako [6]
Total Authors: 6
Document type: Journal article
Source: Applied Biochemistry and Biotechnology; v. 110, n. 2, p. 91-100, Aug. 2003.
Field of knowledge: Biological Sciences - Microbiology
Abstract

Haemophilus influenzae type b (Hib) causes invasive infections in infants and young children. Vaccines consisting of Hib capsular polysaccharide (polymer of ribosylribitol phosphate [PRP]) conjugated to a protein are effective in the prevention of such infections. The production of capsular polysaccharide type b was studied in three cultivation conditions: single, glucose pulse, and repeated batch. Specific polysaccharide production (Y p/x ) was calculated for all experiments, showing the following values: 67 (single-batch cultivation), 71 (glucose pulse), 75 (repeated-batch cultivation, first batch), and 87 mg of PRP/g of dry cell weight (DCW) (repeated-batch cultivation, second batch). Biomass concentration reached ~1.8 g of DCW/L, while polysaccharide concentration was about ~132 mg/L in the three fermentation runs. Polysaccharide synthesis is associated with cell growth in all studied conditions as established by Kono's analysis and Luedeking-Piret's model. (AU)