Early modulation of inflammation by mesenchymal stem cell after acute kidney injury

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Semedo‚ P. ; Palasio‚ C.G. ; Oliveira‚ C.D. ; Feitoza‚ C.Q. ; Gon{\=e}alves‚ G.M. ; Cenedeze‚ M.A. ; Wang‚ P.M.H. ; Teixeira‚ V. ; Reis‚ M.A. ; Pacheco-Silva‚ A. ; others

• Tipo de documento: Artigo Científico
• Fonte: International Immunopharmacology; v. 9, n. 6, p. 677-682, 2009.

Resumo

Therapy with stem cells has showed to be promising for acute kidney injury (AKI), although how it works is still controversial. Modulation of the inflammatory response is one possible mechanism. Most of published data relies on early time and whether the protection is still maintained after that is not known. Here, we analyzed whether immune modulation continues after 24 h of reperfusion. MSC were obtained from male Wistar rats. After 3-5 passages, cells were screened for CD73, CD90, CD44, CD45, CD29 and CD 31. In addition, MSC were submitted to differentiation in adipocyte and in osteocyte. AKI was induced by bilaterally clamping of renal pedicles for 60 min. Six hours after injury, MSC (2 x 105 cells) were administered intravenously. MSC-treated animals presented the lowest serum creatinine compared to non-treated animals (24 h: 1.3 +/- 0.21 vs. 3.23 +/- 0.89 mg/dl, p<0.05). The improvement in renal function was followed by a lower expression of IL-1b, IL-6 and TNF-alpha and higher expression of IL-4 and IL-10. However, 48 h after reperfusion, this cytokine profile has changed. The decrease in Th1 cytokines was less evident and IL-6 was markedly up regulated. PCNA analysis showed that regeneration occurs faster in kidney tissues of MSC-treated animals than in controls at 24 h. And also ratio of Bcl-2/Bad was higher at treated animals after 24 and 48 h. Our data demonstrated that the immunomodulatory effects of MSC occur at very early time point, changing the inflammation profile toward a Th2 profile. (C) 2009 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 06/00620-5 - Análise dos células tronco mesenquimais em modelos de isquemia renal aguda e crônica
• Beneficiário: Patricia Semedo Kuriki
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 04/13826-5 - Contribuição ao aumento da sobrevida do enxerto em transplante de rim: papel dos mecanismos imunológicos e não imunológicos na fisiopatogenia da insuficiência renal aguda
• Beneficiário: Niels Olsen Saraiva Câmara
• Modalidade de apoio: Auxílio à Pesquisa - Regular