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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Raman spectroscopy study of atherosclerosis in human carotid artery

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Autor(es):
Nogueira, Grazielle V. ; Silveira Junior, Landulfo ; Martin, Airton Abrahão [3] ; Zângaro, Renato A. ; Pacheco, Marcos T. T. ; Chavantes, Maria C. ; Pasqualucci, Carlos A.
Número total de Autores: 7
Tipo de documento: Artigo Científico
Fonte: Journal of Biomedical Optics; v. 10, n. 3, p. 00-00, May-June 2005.
Área do conhecimento: Engenharias - Engenharia Biomédica
Assunto(s):Espectroscopia Raman   Aterosclerose   Artérias carótidas
Resumo

Fourier-transform (FT)-Raman spectroscopy has been used for identification and evaluation of human artherosclerotic lesions, providing biochemical information on arteries. In this work, fragments of human carotid arteries postmortem were analyzed using a FT-Raman spectrometer operating at an excitation wavelength of 1064 nm, power of 200 mW, and spectral resolution of 4 cm-1. A total of 75 carotid fragments were spectroscopically scanned and FT-Raman results were compared with histopathology. Discriminant analysis using Mahalanobis distance was applied over principal components scores for tissue classification into three categories: nonatherosclerotic, atherosclerotic plaque without calcification and with calcification. Nonatherosclerotic artery, atherosclerotic plaque, and calcified plaque exhibit spectral signatures related to biochemicals presented in each tissue type, such as bands of collagen and elastin (proteins), cholesterol and its esters, and calcium hydroxyapatite and carbonate apatite, respectively. Spectra of nonatherosclerotic artery were then classified into two groups: normal and discrete diffuse thickening of the intima layer (first group) and moderate and intense diffuse thickening of the intima layer (second group). FT-Raman could identify and classify the tissues found in the atherosclerotic process in human carotid in vitro and had the ability to identify alterations to the diffuse thickening of the intima layer and classify it depending on the intensity of the thickening. (AU)

Processo FAPESP: 01/14384-8 - Estudo de células neoplásicas por espectroscopia de infravermelho próximo
Beneficiário:Airton Abrahao Martin
Linha de fomento: Auxílio à Pesquisa - Regular