Genetic diversity of the E Protein of Dengue Type 3 Virus

Autor(es)

Amarilla, Alberto A. ^[1] ; de Almeida, Flavia T. ^[2] ; Jorge, Daniel M. ^[3] ; Alfonso, Helda L. ^[1] ; de Castro-Jorge, Luiza A. ^[1] ; Nogueira, Nadia A. ^[4] ; Figueiredo, Luiz T. ^[1] ; Aquino, Victor H. ^[2]

Afiliação do(s) autor(es)

^[1] USP, Sch Med Ribeirao Preto, Virol Res Ctr, Ribeirao Preto, SP - Brazil

^[2] USP, FCFRP, Dept Clin Toxicol & Bromatol Anal, Ribeirao Preto, SP - Brazil

^[3] USP, Sch Med Ribeirao Preto, Dept Genet, Bioinformat Lab, Ribeirao Preto, SP - Brazil

^[4] Univ Fed Ceara, Dept Toxicol & Clin Anal, Fortaleza, Ceara - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VIROLOGY JOURNAL; v. 6, p. 113, 2009.
• Citações Web of Science: 23

Resumo

Background: Dengue is the most important arbovirus disease in tropical and subtropical countries. The viral envelope ( E) protein is responsible for cell receptor binding and is the main target of neutralizing antibodies. The aim of this study was to analyze the diversity of the E protein gene of DENV-3. E protein gene sequences of 20 new viruses isolated in Ribeirao Preto, Brazil, and 427 sequences retrieved from GenBank were aligned for diversity and phylogenetic analysis. Results: Comparison of the E protein gene sequences revealed the presence of 47 variable sites distributed in the protein; most of those amino acids changes are located on the viral surface. The phylogenetic analysis showed the distribution of DENV-3 in four genotypes. Genotypes I, II and III revealed internal groups that we have called lineages and sub-lineages. All amino acids that characterize a group (genotype, lineage, or sub-lineage) are located in the 47 variable sites of the E protein. Conclusion: Our results provide information about the most frequent amino acid changes and diversity of the E protein of DENV-3. (AU)

• Processo FAPESP: 05/04178-2 - Construção de clone infeccioso do dengue tipo 3: análises do gene da proteína E
• Beneficiário: Alberto Anastacio Amarilla Ortiz
• Modalidade de apoio: Bolsas no Brasil - Mestrado