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(Reference retrieved automatically from Google Scholar through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genetic diversity of the E Protein of Dengue Type 3 Virus

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Author(s):
Amarilla, Alberto A. [1] ; de Almeida, Flavia T. [2] ; Jorge, Daniel M. [3] ; Alfonso, Helda L. [1] ; de Castro-Jorge, Luiza A. [1] ; Nogueira, Nadia A. [4] ; Figueiredo, Luiz T. [1] ; Aquino, Victor H. [2]
Total Authors: 8
Affiliation:
[1] USP, Sch Med Ribeirao Preto, Virol Res Ctr, Ribeirao Preto, SP - Brazil
[2] USP, FCFRP, Dept Clin Toxicol & Bromatol Anal, Ribeirao Preto, SP - Brazil
[3] USP, Sch Med Ribeirao Preto, Dept Genet, Bioinformat Lab, Ribeirao Preto, SP - Brazil
[4] Univ Fed Ceara, Dept Toxicol & Clin Anal, Fortaleza, Ceara - Brazil
Total Affiliations: 4
Document type: Journal article
Source: VIROLOGY JOURNAL; v. 6, p. 113, 2009.
Web of Science Citations: 23
Abstract

Background: Dengue is the most important arbovirus disease in tropical and subtropical countries. The viral envelope ( E) protein is responsible for cell receptor binding and is the main target of neutralizing antibodies. The aim of this study was to analyze the diversity of the E protein gene of DENV-3. E protein gene sequences of 20 new viruses isolated in Ribeirao Preto, Brazil, and 427 sequences retrieved from GenBank were aligned for diversity and phylogenetic analysis. Results: Comparison of the E protein gene sequences revealed the presence of 47 variable sites distributed in the protein; most of those amino acids changes are located on the viral surface. The phylogenetic analysis showed the distribution of DENV-3 in four genotypes. Genotypes I, II and III revealed internal groups that we have called lineages and sub-lineages. All amino acids that characterize a group (genotype, lineage, or sub-lineage) are located in the 47 variable sites of the E protein. Conclusion: Our results provide information about the most frequent amino acid changes and diversity of the E protein of DENV-3. (AU)

FAPESP's process: 05/04178-2 - Dengue type 3 infectious clone construction: E protein gene analysis
Grantee:Alberto Anastacio Amarilla Ortiz
Support Opportunities: Scholarships in Brazil - Master