Effects of different cryopreservation methods on post-thaw culture conditions of in vitro produced bovine embryos

Autor(es)

Nicacio, Alessandra Corallo ^{[1, 2]} ; Simoes, Renata ^[1] ; de Paula-Lopes, Fabiola Freitas ^[1] ; Oliveira de Barros, Flavia Regina ^[1] ; Peres, Maria Angelica ^[1] ; Ortiz D'Avila Assumpcao, Mayra Elena ^[1] ; Visintin, Jose Antonio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Coll Vet Med & Anim Sci, Dept Anim Reprod, BR-05508270 Sao Paulo - Brazil

^[2] Univ Estadual Maranhao, Coll Vet Med, Sao Luis - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ZYGOTE; v. 20, n. 2, p. 117-122, MAY 2012.
• Citações Web of Science: 8

Resumo

The aim of this work was to evaluate the effect of cryopreservation protocols on subsequent development of in vitro produced bovine embryos under different culture conditions. Expanded in vitro produced blastocysts (n = 600) harvested on days 7-9 were submitted to controlled freezing {[}slow freezing group: 10% ethylene glycol (EG) for 10 min and 1.2 degrees C/min cryopreservation]; quick-freezing {[}rapid freezing group: 10% EG for 10 min, 20% EG + 20% glycerol (Gly) for 30 s]; or vitrification {[}vitrification group: 10% EG for 10 min, 25% EG + 25% Gly for 30 s] protocols. Control group embryos were not exposed to cryoprotectant or cryopreservation protocols and the hatching rate was evaluated on day 12 post-insemination. In order to evaluate development, frozen-thawed embryos were subjected to granulosa cell co-culture in TCM199 or SOFaa for 4 days. Data were analyzed by PROC MIXED model using SAS Systems for Windows (R). Values were significant at p < 0.05. The hatching rate of the control group was 46.09%. In embryos cultured in TCM199, slow freezing and vitrification group hatching rates were 44.65 +/- 5.94% and 9.43 +/- 6.77%, respectively. In embryos cultured in SOFaa, slow freezing and vitrification groups showed hatching rates of 11.65 +/- 3.37 and 8.67 +/- 4.47%, respectively. In contrast, the rapid freezing group embryos did not hatch, regardless of culture medium. The slow freezing group showed higher hatching rates than other cryopreservation groups. Under such conditions, controlled freezing (1.2 degrees C/min) can be an alternative to cryopreservation of in vitro produced bovine embryos. (AU)

• Processo FAPESP: 04/05335-1 - Avaliacao do desenvolvimento apos a criopreservacao de embrioes bovinos produzidos in vitro.
• Beneficiário: Alessandra Corallo Nicacio
• Modalidade de apoio: Bolsas no Brasil - Doutorado