High-risk human papillomavirus in oral squamous cell carcinoma of young patients

Autor(es)

Kaminagakura, Estela ^[1] ; Villa, Luisa Lina ^[2] ; Andreoli, Maria Antonieta ^[2] ; Sobrinho, Joao Simao ^[2] ; Vartanian, Jose Guilherme ^[1] ; Soares, Fernando Augusto ^[3] ; Nishimoto, Ines Nobuko ^[1] ; Rocha, Rafael ^[3] ; Kowalski, Luiz Paulo ^[1]

Afiliação do(s) autor(es)

^[1] AC Camargo Hosp, Dept Head & Neck Surg & Otorhinolaryngol, BR-01509900 Sao Paulo - Brazil

^[2] Ludwig Inst Canc Res, Sao Paulo - Brazil

^[3] AC Camargo Hosp, Dept Anat Pathol, BR-01509900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Cancer; v. 130, n. 8, p. 1726-1732, APR 15 2012.
• Citações Web of Science: 34

Resumo

The aim of this study was to investigate a possible relation between oral squamous cell carcinoma (SCC), the presence of high-risk human papillomavirus (HR-HPV) DNA and p16 expression in young patients. Paraffin-embedded tumor blocks from 47 oral SCC of young (<= 40-year old) patients were evaluated. The presence of HPV DNA in tumor specimens was analyzed by polymerase chain reaction (PCR) using GP5+/GP6+ generic primers (L1 region) followed by dot blot hybridization for HPV typing. When necessary, the HPV16 positivity was confirmed by PCR HPV16 E7-specific primers. Cases involving young patients were compared with 67 oral SCC from patients >= 50-year old (controls). Demographic and clinical data were collected to analyze patient outcomes. p16(ink4) expression was evaluated by immunostaining of tissue microarrays. HPV16 was detected in 22 (19.2%) cases; 15 (68.2%) young and 7 (31.8%) control patients, a statistically significant difference (p = 0.01). In 1 (1.7%) young group specimen, HPV DNA 16 and 18 was detected. p16 expression was observed in 11 (25.6%) cases from the young group and in 11 (19.6%) controls (p = 0.48). Association between HPV and p16 was verified, and it was statistically significant (p = 0.002). The higher prevalence of high-risk HPV types, especially HPV16, may be a contributing factor to oral carcinogenesis in younger individuals. (AU)

• Processo FAPESP: 07/56117-2 - Aspectos clinicopatológicos e moleculares do carcinoma epidermóide bucal em pacientes com idade menor ou igual a 40 anos
• Beneficiário: Luiz Paulo Kowalski
• Modalidade de apoio: Auxílio à Pesquisa - Regular