Intrauterine food restriction alters the expression of uncoupling proteins in brown adipose tissue of rat newborns

Autor(es)

Souza, Thais L. V. ^[1] ; Coelho, Carolina T. ^[1] ; Guimaraes, Paola B. ^[1] ; Goto, Eduardo M. ^[2] ; Silva, Sylvia Maria A. ^[1] ; Silva, Jr., Jose Antonio ^[3] ; Nunes, Maria Tereza ^[4] ; Ihara, Silvia S. M. ^[2] ; Luz, Jacqueline ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Physiol, BR-04023900 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Pathol, BR-04023900 Sao Paulo - Brazil

^[3] Univ Nove Julho, Dept Rehabil Sci, UNINOVE, BR-05001100 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Dept Physiol & Biophys, BR-05508000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Thermal Biology; v. 37, n. 2, p. 138-143, FEB 2012.
• Citações Web of Science: 1

Resumo

A previous study from our laboratory showed that maternal food restriction (MFR) delays thermoregulation in newborn rats. In neonates brown adipose tissue (BAT) is essential for thermogenesis due to the presence of uncoupling proteins (UCPs). The aim of this study was to evaluate the influence of MFR on the UCPs mRNA and protein expression in BAT and skeletal muscle (SM) of the newborn rat. Female Wistar EPM-1 control rats (CON) received chow ad libitum during pregnancy, whereas food-restricted dams (RES) received 50% of the amount ingested by CON. Fifteen hours after birth, the litters were weighed and sacrificed. Blood was collected for hormonal analysis. BAT and SM were used for determination of UCPs mRNA and protein expression, and Ca2+-ATPase sarcoplasmic reticulum (SERCA1). RES pups showed a significant reduction in body weight and fat content at birth. MFR caused a significant increase in the expression of UCP1 and UCP2 in BAT, without changes in UCP3 and SERCA1 expression in BAT and SM. No differences between groups were found for leptin, T4 and glucose levels. RES pups showed increased insulin and decreased T3 levels. The delay in development of thermoregulation previously described in RES animals appears not to result from impairment in thermogenesis, but from an increase in heat loss, since MFR caused low birth weight in pups, leading to greater surface/volume ratio. The higher expression of UCP1 and UCP2 in BAT suggests a compensatory mechanism to increased thermogenesis. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 04/14749-4 - Efeito da restricao alimentar intra-uterina sobre o desenvolvimento da termorregulacao da prole.
• Beneficiário: Jacqueline Luz
• Modalidade de apoio: Auxílio à Pesquisa - Regular