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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Detection of human T-cell lymphotropic virus type 1 in plasma samples

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Autor(es):
Cabral, Fabio [1] ; Arruda, Lia Barbara [2] ; de Araujo, Marilia Ladeira [1] ; Montanheiro, Patricia [1] ; Smid, Jerusa ; Penalva de Oliveira, Augusto Cesar ; Duarte, Alberto J. S. [1] ; Casseb, Jorge [1, 2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Med, Lab Immunodeficiencies & Dermatol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Trop Med Inst Sao Paulo, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: VIRUS RESEARCH; v. 163, n. 1, p. 87-90, JAN 2012.
Citações Web of Science: 13
Resumo

Human T-cell lymphotropic virus type 1 (HTLV-1) is-an RNA virus responsible for diseases such as HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) and adult T-cell leukemia/lymphoma (ATL). Cell-to-cell contact and Tax-induced clonal expansion of infected cells are the main modes of virus replication, making virus detection during the viremic stage difficult. Consequently, the proviral load is the current virologic marker for disease monitoring, but the mechanisms of progression have not been established yet. Thus, this study investigated the presence of virus in plasma from asymptomatic HTLV-1 carriers and from HAM/TSP patients. Real-time PCR was performed on DNA from 150 plasma samples; 12(8%) had detectable DNA amplification, including 6(4%) asymptomatic HTLV-1 carriers and 14(26%) HAM/TSP patients (p < 0.005). Of the 33 samples submitted for nested PCR, six (18%, p = 0.02) were positive for HTLV-1 RNA in the plasma. Additionally, 26 plasma samples were treated with DNAse enzyme to eliminate any DNA contamination before RNA extraction. Two of them (8%) showed amplification for HTLV-1 (p = 0.5). Therefore, this study described for the first time the detection of free HTLV-1 RNA in plasma from HTLV-1-infected subjects, regardless of their clinical status. Thus, HTLV-1 viral replication does occur in plasma, and other transmission pathways for HTLV-1 should be investigated further. (C) 2011 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 99/11188-1 - Perfil de citocinas e quantificação de carga viral de DNA de HTLV-I em indivíduos infectados pelo HIV e co-infectados pelo vírus HTLV-I
Beneficiário:Jorge Simão do Rosário Casseb
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 08/56427-4 - Diversidade molecular do envelope e carga proviral do vírus linfotrópico de células T humanas tipo 2 (HTLV-2) em amotras indeterminadas pelo teste western-blot
Beneficiário:Jorge Simão do Rosário Casseb
Linha de fomento: Auxílio à Pesquisa - Regular