Up-regulation of chemokine C-C ligand 2 (CCL2) and C-X-C chemokine 8 (CXCL8) expression by monocytes in chronic idiopathic urticaria

Autor(es)

Santos, J. C. ^[1] ; de Brito, C. A. ^[1] ; Futata, E. A. ^[1] ; Azor, M. H. ^[1] ; Orii, N. M. ^[1] ; Maruta, C. W. ^[2] ; Rivitti, E. A. ^[2] ; Duarte, A. J. S. ^[1] ; Sato, M. N. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Lab Dermatol & Immunodeficiencies LIM 56, Dept Dermatol, BR-05403000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dermatol Outpatient Clin, Hosp Clin, Sch Med, BR-05403000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL AND EXPERIMENTAL IMMUNOLOGY; v. 167, n. 1, p. 129-136, JAN 2012.
• Citações Web of Science: 13

Resumo

The disturbed cytokinechemokine network could play an important role in the onset of diseases with inflammatory processes such as chronic idiopathic urticaria (CIU). Our main objectives were to evaluate the relation between proinflammatory chemokine serum levels from CIU patients and their response to autologous skin test (ASST) and basophil histamine release (BHR). We also aimed to assess the chemokine secretion by peripheral blood mononuclear cells (PBMC) upon polyclonal stimulus and to evaluate chemokine CC ligand 2/C-X-C chemokine 8 (CCL2/CXCL8) and Toll-like receptor-4 (TLR-4) expression in monocytes. We observed significantly higher serum levels of the CXCL8, CXCL9, CXCL10 and CCL2 in CIU patients compared to the healthy group, regardless of the BHR or ASST response. The basal secretion of CCL2 by PBMC or induced by Staphylococcus aureus enterotoxin A (SEA) was higher in CIU patients than in the control group, as well as for CXCL8 and CCL5 secretions upon phytohaemagglutinin stimulation. Also, up-regulation of CCL2 and CXCL8 mRNA expression was found in monocytes of patients upon SEA stimulation. The findings showed a high responsiveness of monocytes through CCL2/CXCL8 expression, contributing to the creation of a proinflammatory environment in CIU. (AU)

• Processo FAPESP: 07/58485-9 - Caracterizacao fenotipica e grau de ativacao de celulas dendriticas plasmocitoides ao estimulo in vitro com oligodeoxinucleotideos cpg na urticaria cronica.
• Beneficiário: Maria Notomi Sato
• Modalidade de apoio: Auxílio à Pesquisa - Regular