Early Changes Induced by Short-Term Low-Dose Cadmium Exposure in Rat Ventral and Dorsolateral Prostates

Autor(es)

Lacorte, Livia M. ; Delella, Flavia K. ; Amorim, Elaine M. Porto ; Justulin, Jr., Luis A. ^[1] ; Godinho, Antonio F. ^[2] ; Almeida, Alaor A. ^[2] ; Pinheiro, Patricia F. Felipe ; Amorim, Renee L. ^[3] ; Felisbino, Sergio L. ^[4]

Afiliação do(s) autor(es)

^[1] Univ Fed Triangulo Mineiro, Uberaba, MG - Brazil

^[2] Univ Estadual Paulista UNESP, IB, CEATOX, BR-18618970 Botucatu, SP - Brazil

^[3] Univ Estadual Paulista UNESP, FMVZ, Clin Vet, BR-18618970 Botucatu, SP - Brazil

^[4] Univ Estadual Paulista UNESP, Dept Morfol, Inst Biociencias, IB, BR-18618970 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROSCOPY RESEARCH AND TECHNIQUE; v. 74, n. 11, p. 988-997, NOV 2011.
• Citações Web of Science: 9

Resumo

Prostate cancer (PCa) is the most commonly diagnosed cancer in men. The etiology of PCa in humans is multifactorial and includes age, ethnicity, environmental factors, and other unknown causes. Epidemiological and experimental evidence has shown that cadmium is associated with PCa both in humans and rodents. This metal can act as an endocrine disruptor during prostate development, and it induces prostate lesions late in life. In this study, we investigated the effects of low-dose cadmium on rat prostate morphology during puberty. Two-month-old male Wistar rats were randomized into two experimental groups: cadmium-treated and control. The ventral and dorsolateral prostates were dissected, weighed, and immunohistochemically stained with specific antibodies against Ki-67 and the androgen receptor (AR). The concentration of cadmium was measured in the blood and prostate, and testosterone concentration was measured from the plasma. Our results show that cadmium concentration was increased in both the blood and the prostate of cadmium-treated rats, but there were no changes in the prostatic weight, epithelial cell height, or testosterone levels. However, AR immunostaining and epithelial cell proliferation (Ki-67 index) were increased in both prostates with an increase in apoptosis only in the dorsal lobe. Furthermore, atypical hyperplasic proliferative lesions were found in the dorsolateral lobe after cadmium exposure. Cadmium treatment reduced collagen fiber absolute volume in both prostates. Thus, low-doses of cadmium, even for a short period of time, can interfere with prostate epithelium-stroma homeostasis, and this disruption might be an important factor in the onset of prostate lesions late in life. Microsc. Res. Tech. 74: 988-997, 2011. (C) 2011 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 06/60115-2 - Efeito do diabete e da reposição com insulina sobre o processo de maturação da próstata ventral do rato Wistar: alterações morfológicas e na atividade de metaloproteinases de matriz MMP-2 e -9
• Beneficiário: Sérgio Luis Felisbino
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/54265-1 - Efeitos da cafeína sobre as lesões induzidas pelo cádmio na próstata ventral do rato Wistar: proliferação e morte celular
• Beneficiário: Lívia Maria Lacorte
• Linha de fomento: Bolsas no Brasil - Mestrado