Increased oxidative stress in the placenta tissue and cell culture of tumour-bearing pregnant rats

Autor(es)

Toledo, M. T. ^[1] ; Ventrucci, G. ^[1] ; Gomes-Marcondes, M. C. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Inst Biol, Dept Physiol & Biophys, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Placenta; v. 32, n. 11, p. 859-864, NOV 2011.
• Citações Web of Science: 7

Resumo

Placental dysfunction leads to foetal damage, which jeopardises the exchange between the maternal and foetal systems. We evaluated the effects of tumour growth on the activity of antioxidant enzymes and oxidative stress in placental tissue and cell culture from tumour-bearing pregnant rats compared to non-tumour-bearing pregnant rats that were ascitic fluid injected. Ascitic fluid is obtained from Walker tumour-bearing rats and contains a cytokine called Walker factor (WF), which is a molecule similar to proteolysis-inducing factor (PIF), and induces changes in protein metabolism and oxidative stress. Pregnant Wistar rats were distributed into control (C), tumour-bearing (W) and ascitic fluid injected (A) groups and were sacrificed on days 16,19 and 21 of pregnancy to analyse the profile of enzyme activities (glutathione-S-transferase (GST), catalase (CAT), alkaline phosphatase (AP)) and malondialdehyde (MDA) content in placental tissue. Meanwhile, placenta samples from all groups were obtained on day 21, placed in primary culture and treated with WF for 72 h. The presence of tumour or ascitic fluid reduced the protein content of the placental tissue. On day 16 there was a significant reduction in AP activity in W rats, and on day 19, CAT activity and MDA content significantly increased. These results indicate that the presence of cancer decreased antioxidant enzyme capacity in the placenta, increasing the amount of oxidation in these cells, which may contribute to irreversible placental damage and compromisefoetal development. WF treatment induces similar changes in placental cells in primary culture, resulting in less cell viability and increased oxidative stress. These results indicate that WF, provided by the tumour or inoculation of ascitic fluid, has negative effects on placental homeostasis, which impairs foetal health. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 97/01002-2 - Estudo dos aspectos morfometricos e funcionais da placenta de ratas wistar jovens e adultas, portadoras do carcinossarcoma de walker 256.
• Beneficiário: Mércia Tancredo Toledo
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 04/00514-5 - Crescimento neoplásico associado à gravidez: estudo das alterações metabólicas e moleculares do organismo materno e fetal
• Beneficiário: Maria Cristina Cintra Gomes Marcondes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 96/09463-6 - Correlação entre câncer, gravidez e nutrição: estudo experimental sobre as alterações no metabolismo protéico em ratos jovens
• Beneficiário: Maria Cristina Cintra Gomes Marcondes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 01/02135-3 - Efeito da associação entre nutrição, câncer e gravidez: estudo dos processos metabólicos e moleculares da caquexia in vivo in vitro
• Beneficiário: Maria Cristina Cintra Gomes Marcondes
• Modalidade de apoio: Auxílio à Pesquisa - Regular