Bovine fetal DNA in the maternal circulation: Applications and implications

Autor(es)

Lemos, D. C. ^[1] ; Takeuchi, P. L. ^[1] ; Rios, A. F. L. ^{[1, 2]} ; Araujo, A. ^[1] ; Lemos, H. C. ^[1] ; Ramos, E. S. ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Genet, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, Campos Dos Goytacazes, RJ - Brazil

^[3] Univ Sao Paulo, Dept Ginecol & Obstet, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Placenta; v. 32, n. 11, p. 912-913, NOV 2011.
• Citações Web of Science: 13

Resumo

Objective: The main aim of the present study was to detect bovine fetal DNA in the maternal circulation, a relatively unexplored subject in the literature. Study design: DNA was extracted from blood of 84 primipara cows (Bos indicus) at different gestational ages (30-270 days) and from 100 adult animals (50 males and 50 non-pregnant cows). The samples were analyzed using PCR with primers for TSPY gene. Results: Molecular results matched the fetal phenotypic gender in all 47 male and 37 female fetuses, including early pregnancy, and in control animals. Conclusions: These results evidence a bovine transplacental fetal DNA passage. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 09/08313-2 - Expressão diferencial e padrão de metilação da região MHM durante o desenvolvimento gonadal de galinhas
• Beneficiário: Ester Silveira Ramos
• Modalidade de apoio: Auxílio à Pesquisa - Regular