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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Metabolic fingerprinting using direct flow injection electrospray ionization tandem mass spectrometry for the characterization of proanthocyanidins from the barks of Hancornia speciosa

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Autor(es):
Rodrigues, Clenilson M. ; Rinaldo, Daniel ; Santos, Lourdes C. dos ; Montoro, Paola ; Piacente, Sonia ; Pizza, Cosimo ; Hiruma-Lima, Clélia A. ; Brito, Alba R. M. Souza ; Vilegas, Wagner [9]
Número total de Autores: 9
Tipo de documento: Artigo Científico
Fonte: Rapid Communications in Mass Spectrometry; v. 21, n. 12, p. 1907-1914, June 2007.
Área do conhecimento: Ciências Exatas e da Terra - Química
Assunto(s):Fitoterapia   Plantas medicinais   Apocynaceae   Úlcera gástrica   Espectrometria de massas
Resumo

Direct flow injection electrospray ionization ion trap tandem mass spectrometry (ESI-IT-MS/MS) was used to investigate the polyphenolic compounds present in an infusion from the barks of Hancornia speciosa Gom. (Apocynaceae), a native Brazilian plant popularly known as 'mangabeira', used as a source of nutrition and against gastric disorders. After a simple sample filtration pretreatment the characteristic fingerprint of the infusion was performed in negative ion ESI mode in a few minutes. At low capillary-voltage activation, the deprotonated molecules ([M-H]-) were observed and using collision-induced dissociation the product ion spectra showed the presence of a homologous series of B-type proanthocyanidins, as well as another series containing their respective C-glycosylated derivatives, with a degree of polymerization from 1 up to 6 units of interlinked catechins. Therefore, direct flow injection allowed us to identify the key compounds without preparative isolation of the components. (AU)

Processo FAPESP: 02/05503-6 - Uso sustentável da biodiversidade brasileira: prospecção químico-farmacológica em plantas superiores
Beneficiário:Wagner Vilegas
Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático