Metabolic fingerprinting using direct flow injection electrospray ionization tandem mass spectrometry for the characterization of proanthocyanidins from the barks of Hancornia speciosa

Autor(es)

Rodrigues, Clenilson M. ; Rinaldo, Daniel ; Santos, Lourdes C. dos ; Montoro, Paola ; Piacente, Sonia ; Pizza, Cosimo ; Hiruma-Lima, Clélia A. ; Brito, Alba R. M. Souza ; Vilegas, Wagner ^[9]

• Tipo de documento: Artigo Científico
• Fonte: Rapid Communications in Mass Spectrometry; v. 21, n. 12, p. 1907-1914, June 2007.
• Área do conhecimento: Ciências Exatas e da Terra - Química
• Assunto(s): Fitoterapia Plantas medicinais Apocynaceae Úlcera gástrica Espectrometria de massas

Resumo

Direct flow injection electrospray ionization ion trap tandem mass spectrometry (ESI-IT-MS/MS) was used to investigate the polyphenolic compounds present in an infusion from the barks of Hancornia speciosa Gom. (Apocynaceae), a native Brazilian plant popularly known as 'mangabeira', used as a source of nutrition and against gastric disorders. After a simple sample filtration pretreatment the characteristic fingerprint of the infusion was performed in negative ion ESI mode in a few minutes. At low capillary-voltage activation, the deprotonated molecules ([M-H]-) were observed and using collision-induced dissociation the product ion spectra showed the presence of a homologous series of B-type proanthocyanidins, as well as another series containing their respective C-glycosylated derivatives, with a degree of polymerization from 1 up to 6 units of interlinked catechins. Therefore, direct flow injection allowed us to identify the key compounds without preparative isolation of the components. (AU)

• Processo FAPESP: 02/05503-6 - Uso sustentável da biodiversidade brasileira: prospecção químico-farmacológica em plantas superiores
• Beneficiário: Wagner Vilegas
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático