Prospective evaluation of HSV, Candida spp., and oral bacteria on the severity of oral mucositis in pediatric acute lymphoblastic leukemia

Autor(es)

Holanda de Mendonca, Regina Maria ^[1] ; de Araujo, Marcela ^[2] ; Levy, Carlos Emilio ^[3] ; Morari, Joseane ^[4] ; Silva, Rosangela A. ^[5] ; Yunes, Jose Andres ^[2] ; Brandalise, Silvia Regina ^[6]

Afiliação do(s) autor(es)

^[1] Ctr Infantil Boldrini, Unidade Odontol, BR-13083210 Campinas, SP - Brazil

^[2] Ctr Infantil Boldrini, Lab Biol Mol, BR-13083210 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Dept Patol Clin, Campinas, SP - Brazil

^[4] Univ Estadual Campinas, Inst Biol, Campinas, SP - Brazil

^[5] Ctr Infantil Boldrini, Microbiol Lab, BR-13083210 Campinas, SP - Brazil

^[6] Univ Estadual Campinas, Dept Pediat, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SUPPORTIVE CARE IN CANCER; v. 20, n. 5, p. 1101-1107, MAY 2012.
• Citações Web of Science: 20

Resumo

Background Oral mucositis is a common collateral effect among the secondary complications resulting from chemotherapy. The objective of this study was to prospectively evaluate the association of HSV-1, Candida spp., and oral bacteria on the severity of oral mucositis in pediatric acute lymphoblastic leukemia (ALL). Procedure Seventy-one prospective patients were included. Analyses of oral microbiota were conducted on days 14 (D14) and 56 (D56) of the Brazilian GBTLI-99 treatment protocol. Herpes simplex virus (HSV) identification was performed by PCR followed by DNA sequencing analysis. Bacteria and fungi identification was obtained by standard microbiological culture tests. Results HSV-1 was found in 10.37% of individual patient samples. One sample was positive for HSV-4. On D14, we found an association between the severity of mucositis and the presence of HSV (p=0.0347) and Candida spp. (p=0.0078). At D56, we found an association between the severity of mucositis and the presence of HSV on D14 (p<0.0001) and HSV presence (p=0.0317). Conclusion The presence of HSV, mainly HSV-1, and Candida spp. was associated with mucositis severity in pediatric ALL. No association could be found between bacterial CFU and severity of mucositis. (AU)

• Processo FAPESP: 04/11274-5 - Analise da associacao entre elementos virais da microbiota oral e a intensidade da mucosite oral em pacientes pediatricos portadores de leucemia linfoide aguda subemtidos ao tratamento antineoplasico.
• Beneficiário: Silvia Regina Brandalise
• Modalidade de apoio: Auxílio à Pesquisa - Regular