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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Prospective evaluation of HSV, Candida spp., and oral bacteria on the severity of oral mucositis in pediatric acute lymphoblastic leukemia

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Author(s):
Holanda de Mendonca, Regina Maria [1] ; de Araujo, Marcela [2] ; Levy, Carlos Emilio [3] ; Morari, Joseane [4] ; Silva, Rosangela A. [5] ; Yunes, Jose Andres [2] ; Brandalise, Silvia Regina [6]
Total Authors: 7
Affiliation:
[1] Ctr Infantil Boldrini, Unidade Odontol, BR-13083210 Campinas, SP - Brazil
[2] Ctr Infantil Boldrini, Lab Biol Mol, BR-13083210 Campinas, SP - Brazil
[3] Univ Estadual Campinas, Dept Patol Clin, Campinas, SP - Brazil
[4] Univ Estadual Campinas, Inst Biol, Campinas, SP - Brazil
[5] Ctr Infantil Boldrini, Microbiol Lab, BR-13083210 Campinas, SP - Brazil
[6] Univ Estadual Campinas, Dept Pediat, Campinas, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: SUPPORTIVE CARE IN CANCER; v. 20, n. 5, p. 1101-1107, MAY 2012.
Web of Science Citations: 20
Abstract

Background Oral mucositis is a common collateral effect among the secondary complications resulting from chemotherapy. The objective of this study was to prospectively evaluate the association of HSV-1, Candida spp., and oral bacteria on the severity of oral mucositis in pediatric acute lymphoblastic leukemia (ALL). Procedure Seventy-one prospective patients were included. Analyses of oral microbiota were conducted on days 14 (D14) and 56 (D56) of the Brazilian GBTLI-99 treatment protocol. Herpes simplex virus (HSV) identification was performed by PCR followed by DNA sequencing analysis. Bacteria and fungi identification was obtained by standard microbiological culture tests. Results HSV-1 was found in 10.37% of individual patient samples. One sample was positive for HSV-4. On D14, we found an association between the severity of mucositis and the presence of HSV (p=0.0347) and Candida spp. (p=0.0078). At D56, we found an association between the severity of mucositis and the presence of HSV on D14 (p<0.0001) and HSV presence (p=0.0317). Conclusion The presence of HSV, mainly HSV-1, and Candida spp. was associated with mucositis severity in pediatric ALL. No association could be found between bacterial CFU and severity of mucositis. (AU)