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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

In vitro cytotoxicity of Selol-loaded magnetic nanocapsules against neoplastic cell lines under AC magnetic field activation

Texto completo
Autor(es):
Falqueiro, A. M. [1, 2] ; Siqueira-Moura, M. P. [1, 2] ; Jardim, D. R. [2] ; Primo, F. L. [2, 1] ; Morais, P. C. [3] ; Mosiniewicz-Szablewska, E. [4] ; Suchocki, P. [5, 6] ; Tedesco, A. C. [2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Lab Fotobiol & Fotomed, BR-14040901 Ribeirao Preto, SP - Brazil
[3] Univ Brasilia, Inst Fis, Nucleo Fis Aplicada, BR-70910900 Brasilia, DF - Brazil
[4] Polish Acad Sci, Inst Phys, PL-02668 Warsaw - Poland
[5] Warsaw Med Univ, Dept Drugs Anal, PL-02097 Warsaw - Poland
[6] Drug Inst, Dept Pharmaceut Chem, PL-00725 Warsaw - Poland
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: Journal of Applied Physics; v. 111, n. 7 APR 1 2012.
Citações Web of Science: 8
Resumo

The goals of this study are to evaluate in vitro compatibility of magnetic nanomaterials and their therapeutic potential against cancer cells. Highly stable ionic magnetic fluid sample (maghemite, gamma-Fe2O3) and Selol were incorporated into polymeric nanocapsules by nanoprecipitation method. The cytotoxic effect of Selol-loaded magnetic nanocapsules was assessed on murine melanoma (B16-F10) and oral squamous cell carcinoma (OSCC) cell lines following AC magnetic field application. The influence of different nanocapsules on cell viability was investigated by colorimetric MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. In the absence of AC magnetic field Selol-loaded magnetic nanocapsules, containing 100 mu g/mL Selol plus 5 x 10(12) particle/mL, showed antitumoral activity of about 50% on B16-F10 melanoma cells while OSCC carcinoma cells demonstrated drug resistance at all concentrations of Selol and magnetic fluid (range of 100-500 mu g/mL Selol and 5 x 10(12) -2.5 x 10(13) particle/mL). On the other hand, under AC applied fields (1 MHz and 40 Oe amplitude) B16-F10 cell viability was reduced down to 40.5% (+/- 3.33) at the highest concentration of nanoencapsulated Selol. The major effect, however, was observed on OSCC cells since the cell viability drops down to about 33.3% (+/- 0.38) under application of AC magnetic field. These findings clearly indicate that the Selol-loaded magnetic nanocapsules present different toxic effects on neoplastic cell lines. Further, the cytotoxic effect was maximized under AC magnetic field application on OSCC, which emphasizes the effectiveness of the magnetohyperthermia approach. (C) 2012 American Institute of Physics. {[}doi: 10.1063/1.3680541] (AU)

Processo FAPESP: 08/53719-4 - Utilização de nanocarreadores contendo fármacos fotossensíveis e outros ativos derivados de fatores de crescimento aplicados a processos fotodinâmicos nas áreas da saúde
Beneficiário:Antonio Claudio Tedesco
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 09/15363-6 - Desenvolvimento e caracterização de equivalentes dérmicos modificados com células tronco mesenquimais como modelos para avaliação de matriz extracelular após fotoestímulo com diodo-laser de baixa potência
Beneficiário:Fernando Lucas Primo
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 09/13208-3 - Preparação, caracterização e avaliação da atividade antitumoral in vitro de nanocápsulas contendo Selol
Beneficiário:André Miotello Falqueiro
Linha de fomento: Bolsas no Brasil - Mestrado