Hepatoprotective and anti-inflammatory effects of silibinin on experimental preeclampsia induced by 1-NAME in rats

Autor(es)

de Souza, Camila Oliveira ^[1] ; Serrao Peracoli, Maria Terezinha ^[2] ; Weel, Ingrid Cristina ^[1] ; Bannwart, Camila Ferreira ^[1] ; Romao, Mariana ^[1] ; Nakaira-Takahagi, Erika ^[2] ; Lopes de Medeiros, Leonardo Teixeira ^[1] ; da Silva, Marcia Guimaraes ^[1] ; Peracoli, Jose Carlos ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, Botucatu Med Sch, Dept Gynecol & Obstet, BR-18618970 Botucatu, SP - Brazil

^[2] Sao Paulo State Univ, UNESP, Inst Biosci, Dept Microbiol & Immunol, BR-18618970 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Life Sciences; v. 91, n. 5-6, p. 159-165, SEP 4 2012.
• Citações Web of Science: 4

Resumo

Aims: Inhibition of nitric oxide synthase with N-omega-nitro-L-arginine methyl ester (L-NAME) has been employed as an experimental model of human preeclampsia. This study determined the protective effect of silibinin, a flavonoid with anti-inflammatory and hepatoprotective properties on the deleterious effects observed in experimentally induced preeclampsia in rats. Main methods: Pregnant Wistar rats were treated during gestation (days 10-20) with L-NAME (70-80 mg/kg/day) in drinking water or with L-NAME plus silibinin (100 mg/kg/day, orally) starting at day 0, day 7 or day 14 of pregnancy. Systolic blood pressure was recorded from gestation days 0 to 21. A control group of pregnant non-treated rats was analyzed similarly. On day 21 the rats were euthanized and the following parameters were evaluated: proteinuria, platelet count, liver histopathology and reproductive outcome. Tumor necrosis factor-alpha (TNF-alpha), interleukin-1 (IL-1 beta), IL-6, IL-10 and interferon-gamma (IFN-gamma) were determined in liver homogenate by enzyme immunoassay. Key findings: In comparison with the L-NAME group the silibinin treatment reduced the values of systolic blood pressure, proteinuria, INF-alpha, IL-1 beta and IFN-gamma in liver, normalized the platelet count and improved fetal outcomes. Histopathological lesions in liver of the L-NAME group showed intense mononuclear inflammatory infiltrate and thickening of muscle tunica of arterial vessel, mainly in the periportal area. Silibinin treatment induced attenuation of periportal inflammatory infiltrate, showing an association between inflammatory infiltrate and TNF-alpha, IL-1 beta and IFN-gamma levels in liver homogenate. Significance: Silibinin administration to L-NAME-treated rats displays anti-inflammatory and immunomodulatoty actions that may contribute to its hepatoprotective effects and improve reproductive outcomes in experimental preeclampsia. (C) 2012 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 06/07095-3 - Efeito modulador da silimarina (Silybum marianum) no mecanismo fisiopatológico da pré-eclâmpsia
• Beneficiário: Jose Carlos Peraçoli
• Modalidade de apoio: Auxílio à Pesquisa - Regular