CD44(+)/CD24(-) cells and lymph node metastasis in stage I and II invasive ductal carcinoma of the breast

Autor(es)

Tiezzi, Daniel Guimaraes ^[1] ; Mourao Valejo, Fernando Antonio ^[1] ; Cosinski Marana, Heitor Ricardo ^[1] ; Angotti Carrara, Helio Humberto ^[1] ; Benevides, Luciana ^[2] ; Ribeiro Antonio, Heriton Marcelo ^[1] ; Sicchieri, Renata Danielle ^[1] ; Milanezi, Cristiane Maria ^[2] ; da Silva, Joao Santana ^[2] ; de Andrade, Jurandyr Moreira ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Sch Med, Breast Dis Div, Dept Gynecol & Obstet, BR-14048900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Immunol, BR-14048900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MEDICAL ONCOLOGY; v. 29, n. 3, p. 1479-1485, SEP 2012.
• Citações Web of Science: 12

Resumo

The presence of tumor-initiating cells (CD44(+)/CD24(-)) in solid tumors has been reported as a possible cause of cancer metastasis and treatment failure. Nevertheless, little is know about the presence of CD44(+)/CD24(-) cells within the primary tumor and metastasis. The proportion of CD44(+)/CD24(-) cells was analyzed in 40 samples and in 10 lymph node metastases using flow cytometry phenotyping. Anti-human CD326 (EpCam; FITC), antihuman CD227 (MUC-1; FITC), anti-human CD44 (APC), and anti-human CD24 (PE), anti-ABCG2 (PE), and anti-CXCR4 (PeCy7) were used for phenotype analysis. The mean patient age was 60.5 years (range, 33-87 years); mean primary tumor size (pT) was 1.8 cm (0.5-3.5 cm). The Wilcoxon or Kruskal-Wallis test was used for univariate analyses. Logistic regression was used for multivariate analysis. The median percentage of CD44(+)/CD24(-) cells within primary invasive ductal carcinomas (IDC) was 2.7% (range, 0.2-71.2). In lymph node metastases, we observed a mean of 6.1% (range, 0.07-53.7). The percentage of CD44(+)/CD24(-) cells in IDCs was not associated with age, pT, tumor grade and HER2. We observed a significantly enrichment of CD44(+)/CD24(-) and ABCG2(+) cells in ESA(+) cell population in patients with positive lymph nodes (P = 0.02 and P = 0.04, respectively). Our data suggest that metastatic dissemination is associated with an increase in tumorinitiating cells in stage I and II breast cancer. (AU)

• Processo FAPESP: 08/09294-9 - Transição epitélio-mesenquimal na carcinogênese mamária
• Beneficiário: Daniel Guimarães Tiezzi
• Modalidade de apoio: Auxílio à Pesquisa - Regular