| Full text | |
| Author(s): |
Tiezzi, Daniel Guimaraes
[1]
;
Mourao Valejo, Fernando Antonio
[1]
;
Cosinski Marana, Heitor Ricardo
[1]
;
Angotti Carrara, Helio Humberto
[1]
;
Benevides, Luciana
[2]
;
Ribeiro Antonio, Heriton Marcelo
[1]
;
Sicchieri, Renata Danielle
[1]
;
Milanezi, Cristiane Maria
[2]
;
da Silva, Joao Santana
[2]
;
de Andrade, Jurandyr Moreira
[1]
Total Authors: 10
|
| Affiliation: | [1] Univ Sao Paulo, Ribeirao Preto Sch Med, Breast Dis Div, Dept Gynecol & Obstet, BR-14048900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Immunol, BR-14048900 Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | MEDICAL ONCOLOGY; v. 29, n. 3, p. 1479-1485, SEP 2012. |
| Web of Science Citations: | 12 |
| Abstract | |
The presence of tumor-initiating cells (CD44(+)/CD24(-)) in solid tumors has been reported as a possible cause of cancer metastasis and treatment failure. Nevertheless, little is know about the presence of CD44(+)/CD24(-) cells within the primary tumor and metastasis. The proportion of CD44(+)/CD24(-) cells was analyzed in 40 samples and in 10 lymph node metastases using flow cytometry phenotyping. Anti-human CD326 (EpCam; FITC), antihuman CD227 (MUC-1; FITC), anti-human CD44 (APC), and anti-human CD24 (PE), anti-ABCG2 (PE), and anti-CXCR4 (PeCy7) were used for phenotype analysis. The mean patient age was 60.5 years (range, 33-87 years); mean primary tumor size (pT) was 1.8 cm (0.5-3.5 cm). The Wilcoxon or Kruskal-Wallis test was used for univariate analyses. Logistic regression was used for multivariate analysis. The median percentage of CD44(+)/CD24(-) cells within primary invasive ductal carcinomas (IDC) was 2.7% (range, 0.2-71.2). In lymph node metastases, we observed a mean of 6.1% (range, 0.07-53.7). The percentage of CD44(+)/CD24(-) cells in IDCs was not associated with age, pT, tumor grade and HER2. We observed a significantly enrichment of CD44(+)/CD24(-) and ABCG2(+) cells in ESA(+) cell population in patients with positive lymph nodes (P = 0.02 and P = 0.04, respectively). Our data suggest that metastatic dissemination is associated with an increase in tumorinitiating cells in stage I and II breast cancer. (AU) | |
| FAPESP's process: | 08/09294-9 - Epithelial-mesenchymal transition in breast carcinogenesis |
| Grantee: | Daniel Guimarães Tiezzi |
| Support Opportunities: | Regular Research Grants |