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Immunodetection of ALDH-1 and OCT-4 in canine mammary tumors

Grant number: 13/17997-8
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): January 01, 2014
Effective date (End): December 31, 2014
Field of knowledge:Agronomical Sciences - Veterinary Medicine
Principal Investigator:Geórgia Modé Magalhães
Grantee:Juliana Pistore Ragazzi
Home Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil

Abstract

The cell surface markers ALDH-1 and Oct-4 are related to the identification of cancer stem cells (CSCs). It is estimated that the presence of these cells in tumors are associated with unfavorable outcomes. CSC is a cell capable of self-renewal, have abilities to form and metastasis, and is associated with resistance to chemotherapeutic drugs. Canine mammary tumors in these CSCs were isolated by the techniques of flow cytometry and immunohistochemistry using the surface markers CD44 + / CD24-. Authors report that in human breast cancer marker for CSC, ALDH-1 is more related prognostic factors than the phenotype CD44 + / CD24-. The Oct-4 is another marker of CSC as tested by flow cytometry on cell cultures of canine mammary tumors. Thus, the objective is to detect the presence of CSCs by markers ALDH-1 and Oct-4 in paraffin sections of canine mammary neoplasms and correlate with the degree of malignancy. Will be used 40 samples of canine mammary neoplasms, 10 adenomas, tubular carcinomas grade I, grade II and grade III. The technique of immunohistochemistry to be employed will be the polymer. Knowledge of these cells may provide elements that lead to better understanding of breast carcinogenesis and it is possible to improve the prognosis and treatment of these tumors. At the same time, these results can be used for studies of diseases compared. More important would be the contribution of these findings for veterinary oncology, since there are few studies with CSC in mammary tumors in dogs.