Comparison of Endotoxin Levels Found in Primary and Secondary Endodontic Infections

Autor(es)

Gomes, Brenda P. F. A. ^[1] ; Endo, Marcos S. ^[1] ; Martinho, Frederico C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Restorat Dent, Endodont Div, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENDODONTICS; v. 38, n. 8, p. 1082-1086, AUG 2012.
• Citações Web of Science: 27

Resumo

Introduction: This clinical study was conducted to compare the levels of endotoxins (lipopolysaccharides {[}LPSs]) found in primary and secondary endodontic infections with apical periodontitis by correlating LPS contents with clinical/radiographic findings. In addition, the presence of target gram-negative anaerobic bacteria was also investigated. Methods: Samples were taken from 15 root canals with primary infections and 15 with secondary infections by using paper points. The limulus amebocyte lysate assay was used to quantify endotoxins, and the polymerase chain reaction technique (16S rDNA) was used for bacterial investigation. Results: Endotoxins were detected in 100% of the root canal samples collected from primary (15/15) and secondary (15/15) infections with median values of 7.49 EU/mL and 3.96 EU/mL, respectively (P < .05). The median value of endotoxins found in the presence of clinical symptoms was significantly higher than in asymptomatic teeth with primary infections (P < .05). A positive correlation was found between endotoxin contents and a larger size of the radiolucent area (> 3 mm) (P < .05). Prevotella nigrescens (10/15, 4/15), Fusobacterium nucleatum (5/15, 1/15), Treponema denticola (3/15, 1/15), and Treponema socranskii (5/15, 1/15) were detected in teeth with primary and secondary infections, respectively. P endodontalis was present only in teeth with primary infections (5/15). Conclusions: Teeth with primary endodontic infections had higher contents of endotoxins and a more complex gram-negative bacterial community than teeth with secondary infections. Moreover, the levels of endotoxins were related to the severity of bone destruction in periapical tissues as well as the development of clinical features in teeth with primary infections. (J Endod 2012;38:1082-1086) (AU)

• Processo FAPESP: 11/50051-5 - Avaliacao in vivo do efeito do preparo quimico-mecanico sobre microrganismos e endotoxinas em infeccoes endodonticas persistentes e suscetibilidade antimicrobiana dos enterococcus spp. isolados.
• Beneficiário: Marcos Sergio Endo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/17877-4 - Análise microbiológica, de endoxinas e de citocinas pró-inflamatórias em infecções endodônticas primárias com presença de lesões periapicais
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/19136-1 - Estudo microbiológico e de endotoxinas em canais radiculares com necrose pulpar e lesão periapical e avaliação do seu potencial antigênico contra macrófagos na produção de IL1-beta e TNF-alfa durante a terapia endodôntica
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/57954-8 - Estudo microbiológico, quantificação de endotoxinas e investigação da atividade endotóxica de diferentes LPS bacterianos isolados de canais radiculares de dentes com necrose pulpar e presença de lesão periapiacal
• Beneficiário: Frederico Canato Martinho
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 08/57551-0 - Microbiota dos canais radiculares de dentes com insucesso do tratamento endodôntico e análise da suscetibilidade antimicrobiana e dos fatores de virulência dos Enterococcus faecalis isolados
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/50510-0 - Avaliação in vivo do efeito do preparo químico-mecânico sobre microrganismos e endotoxinas em infecções endodônticas persistentes e suscetibilidade antimicrobiana dos Enterococcus spp. isolados
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular