Discriminating model for diagnosis of basal cell carcinoma and melanoma in vitro based on the Raman spectra of selected biochemicals

Autor(es)

Silveira, Jr., Landulfo ^[1] ; Silveira, Fabricio Luiz ^[1] ; Bodanese, Benito ^[2] ; Zangaro, Renato Amaro ^[1] ; Pacheco, Marcos Tadeu T. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Camilo Castelo Branco UNICASTELO, Inst Biomed Engn, BR-12247004 Sao Paulo - Brazil

^[2] Univ Comunitaria Regiao Chapeco UNOCHAPECO, Hlth Sci Ctr CCS, BR-89809000 Chapeco, SC - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biomedical Optics; v. 17, n. 7 JUL 2012.
• Citações Web of Science: 38

Resumo

Raman spectroscopy has been employed to identify differences in the biochemical constitution of malignant {[}basal cell carcinoma (BCC) and melanoma (MEL)] cells compared to normal skin tissues, with the goal of skin cancer diagnosis. We collected Raman spectra from compounds such as proteins, lipids, and nucleic acids, which are expected to be represented in human skin spectra, and developed a linear least-squares fitting model to estimate the contributions of these compounds to the tissue spectra. We used a set of 145 spectra from biopsy fragments of normal (30 spectra), BCC (96 spectra), and MEL (19 spectra) skin tissues, collected using a near-infrared Raman spectrometer (830 nm, 50 to 200 mW, and 20 s exposure time) coupled to a Raman probe. We applied the best-fitting model to the spectra of biochemicals and tissues, hypothesizing that the relative spectral contribution of each compound to the tissue Raman spectrum changes according to the disease. We verified that actin, collagen, elastin, and triolein were the most important biochemicals representing the spectral features of skin tissues. A classification model applied to the relative contribution of collagen III, elastin, and melanin using Euclidean distance as a discriminator could differentiate normal from BCC and MEL. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). {[}DOI: 10.1117/1.JBO.17.7.077003] (AU)

• Processo FAPESP: 09/01788-5 - Espectroscopia Raman dispersiva utilizando fibras ópticas "Raman probe" aplicada ao diagnóstico de neoplasias na pele e próstata
• Beneficiário: Landulfo Silveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular