| Texto completo | |
| Autor(es): |
Victorio, Sheila C. S.
[1]
;
Cartarozzi, Luciana P.
[1]
;
Hell, Rafaela C. R.
[1]
;
Oliveira, Alexandre L. R.
[1]
Número total de Autores: 4
|
| Afiliação do(s) autor(es): | [1] Univ Campinas UNICAMP, Inst Biol, Dept Anat Cell Biol Physiol & Biophys, BR-13083970 Campinas, SP - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF NEUROINFLAMMATION; v. 9, MAY 7 2012. |
| Citações Web of Science: | 7 |
| Resumo | |
Background: The histocompatibility complex (MHC) class I expression in the central nervous system (CNS) regulates synaptic plasticity events during development and adult life. Its upregulation may be associated with events such as axotomy, cytokine exposition and changes in neuron electrical activity. Since IFN gamma is a potent inducer of the MHC I expression, the present work investigated the importance of this pro-inflammatory cytokine in the synaptic elimination process in the spinal cord, as well as the motor recovery of IFN-/-, following peripheral injury. Methods: The lumbar spinal cords of C57BL/6J (wild type) and IFN gamma(-/-) (mutant) mice, subjected to unilateral sciatic nerve transection, were removed and processed for immunohistochemistry and real time RT-PCR, while the sciatic nerves from animals subjected to unilateral crush, were submitted to immunohistochemistry and electron microscopy for counting of the axons. Gait recovery was monitored using the Cat Walk system. Newborn mice astrocyte primary cultures were established in order to study the astrocytic respose in the absence of the IFN. expression. Results: IFN gamma(-/-) mutant mice showed a decreased expression of MHC I and beta 2-microglobulin mRNA coupled with reduced synaptophysin immunolabelling in the lesioned spinal cord segment. Following unilateral nerve transection, the Iba-1 (ionized calcium binding adaptor molecule 1) and glial fibrillary acid protein (GFAP) reactivities increased equally in both strains. In vitro, the astrocytes demonstrated similar GFAP levels, but the proliferation rate was higher in the wild type mice. In the crushed nerves (distal stump), neurofilaments and p75NTR immunolabeling were upregulated in the mutant mice as compared to the wild type and an improvement in locomotor recovery was observed. Conclusion: The present results show that a lack of IFN. affects the MHC I expression and the synaptic elimination process in the spinal cord. Such changes, however, do not delay peripheral nerve regeneration after nerve injury. (AU) | |
| Processo FAPESP: | 12/06310-9 - Decreased MHC I expression in IFN gamma mutant mice alters synaptic elimination in the spinal cord after peripheral injury |
| Beneficiário: | Alexandre Leite Rodrigues de Oliveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Publicações científicas - Artigo |
| Processo FAPESP: | 09/07443-0 - Regeneração nervosa periférica através da técnica de tubulização do nervo isquiático e tratamento com interferon beta |
| Beneficiário: | Luciana Politti Cartarozzi |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 06/06673-3 - Impacto da ausencia do interferon gama na plasticidade sinaptica apos lesao do nervo isquiatico. |
| Beneficiário: | Sheila Cristina da Silva Victorio |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 10/05370-2 - Importância relativa da reatividade glial e da expressão de MHC classe I na manutenção de circuitos neuronais in vitro |
| Beneficiário: | Rafaela Chitarra Rodrigues Hell |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |