The polysaccharide fraction of Propionibacterium acnes modulates the development of experimental focal segmental glomerulosclerosis

Autor(es):Mostrar menos -

Reis, Vanessa Oliveira ^[1] ; Silva, Janice Costa ^[1] ; Souza, Gabriela Trindade ^[2] ; Semedo, Patricia ^[1] ; Buscariollo, Bruna ^[1] ; Pereira, Rafael Luiz ^[1] ; Cenedeze, Marcos Antonio ^[1] ; Pacheco-Silva, Alvaro ^{[1, 3]} ; Longo-Maugeri, Ieda M. ^[2] ; Saraiva Camara, Niels Olsen ^{[1, 4]} ; Keller, Alexandre Castro ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Med, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Sao Paulo - Brazil

^[3] Inst Israelita Ensino & Pesquisa Albert Einstein, Unidade Transplante Renal, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Dept Imunol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Immunobiology; v. 217, n. 9, p. 831-841, SEP 2012.
• Citações Web of Science: 7

Resumo

The pathogenesis of focal segmental glomerulosclerosis (FSGS) appears to be associated with type-2 cytokines and podocyte dysfunction. In this study, we tested the hypothesis that immunization with the polysaccharide fraction of Propionibacterium acnes (PS), a pro-Th1 agonist, may subvert the type-2 profile and protect podocytes from adriamycin-induced glomerulosclerosis. Adriamycin injection resulted in albuminuria and increased serum creatinine in association with loss of glomerular podocin and podoplanin expression, which is consistent with podocyte dysfunction. Renal tissue analysis revealed the expression of transcripts for GATA3 and fibrogenic-related proteins, such as TGF-beta, tissue inhibitor of metalloproteinase-1 (TIMP-1) and metalloproteinase 9 (MMP9). In association with the expression of fibrogenic transcripts, we observed peri-glomerular expression of a-smooth muscle actin (alpha-SMA), indicating epithelial-to-mesenchymal transition, and increased expression of proliferating cell nuclear antigen (PCNA) in tubular cells, suggesting intense proliferative activity. Previous immunization with PS inhibited albuminuria and serum creatinine in association with the preservation of podocyte proteins and inhibition of fibrogenic transcripts and the expression of alpha-SMA and PCNA proteins. Tissue analysis also revealed that PS treatment induced expression of mRNA for GD3 synthase, which is a glycosiltransferase related to the synthesis of GD3, a ganglioside associated with podocyte physiology. In addition, PS treatment inhibited the influx of inflammatory CD8(pos) and CD11b(pos) cells to kidney tissue. Finally, PS treatment on day 4 post-ADM, a period when proteinuria was already established, was able to improve renal function. Thus, we demonstrate that the PS fraction of P. acnes can inhibit FSGS pathogenesis, suggesting that immunomodulation can represent an alternative approach for disease management. (C) 2011 Elsevier GmbH. All rights reserved. (AU)

• Processo FAPESP: 07/07120-0 - O papel de células T invariantes natural killer no desenvolvimento de glomerulonefrite: mecanismos e perspectivas
• Beneficiário: Alexandre de Castro Keller
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores