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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Triiodothyronine Acutely Stimulates Glucose Transport into L6 Muscle Cells Without Increasing Surface GLUT4, GLUT1, or GLUT3

Texto completo
Autor(es):
Teixeira, Silvania Silva [1] ; Tamrakar, Akhilesh K. [2] ; Goulart-Silva, Francemilson [1] ; Serrano-Nascimento, Caroline [1] ; Klip, Amira [3, 4] ; Nunes, Maria Tereza [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508000 Sao Paulo - Brazil
[2] Cent Drug Res Inst, Div Biochem, Lucknow 226001, Uttar Pradesh - India
[3] Hosp Sick Children, Cell Biol Program, Toronto, ON M5G 1X8 - Canada
[4] Univ Toronto, Dept Biochem, Toronto, ON - Canada
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: THYROID; v. 22, n. 7, p. 747-754, JUL 2012.
Citações Web of Science: 19
Resumo

Background: Thyroid hormones (THs) act genomically to stimulate glucose transport by elevating glucose transporter (Slc2a) expression and glucose utilization by cells. However, nongenomic effects of THs are now emerging. Here, we assess how triiodothyronine (T-3) acutely affects glucose transport and the content of GLUT4, GLUT1, and GLUT3 at the surface of muscle cells, and possible interactions between T-3 and insulin action. Methods: Differentiated L6 myotubes transfected with myc-tagged Slc2a4 (L6-GLUT4myc) or Slc2a1 (L6-GLUT1myc) and wild-type L6 myotubes were studied in the following conditions: control, hypothyroid (Tx), Tx plus T3, Tx plus insulin, and Tx plus insulin and T-3. Results: Glucose uptake and GLUT4 content at the cell surface decreased in the Tx group relative to controls. T-3 treatment for 30 minutes increased glucose transport into L6-GLUT4myc cells without altering surface GLUT4 content, which increased only thereafter. The total amount of GLUT4 protein remained unchanged among the groups studied. The surface GLUT1 content of L6-GLUT1myc cells also remained unaltered after T-3 treatment; however, in these cells glucose transport was not stimulated by T-3. In wild-type L6 cells, although T-3 treatment increased the total amount of GLUT3, it did not change the surface GLUT3 content. Moreover, within 30 minutes, T-3 stimulation of glucose uptake was additive to that of insulin in L6-GLUT4myc cells. As expected, insulin elevated surface GLUT4 content and glucose uptake. However, interestingly, surface GLUT4 content remained unchanged or even dropped with T-3 plus insulin. Conclusions: These data reveal that T-3 rapidly increases glucose uptake in L6-GLUT4myc cells, which, at least for 30 minutes, did not depend on an increment in GLUT4 at the cell surface yet potentiates insulin action. We propose that this rapid T-3 effect involves activation of GLUT4 transporters at the cell surface, but cannot discount the involvement of an unknown GLUT. (AU)

Processo FAPESP: 08/56446-9 - Analise molecular das acoes pos-transcricionais dos hormonios tiroideanos nas celulas beta pancreaticas e suas repercussoes sobre a sintese e secrecao de insulina
Beneficiário:Francemilson Goulart da Silva
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 06/52830-3 - Mecanismos envolvidos na ação não genômica do hormônio tireoideano sobre a expressão e translocação da isoforma 4 do transportador de glicose (GLUT4): estudo no tecido muscular esquelético e adiposo
Beneficiário:Silvania da Silva Teixeira
Modalidade de apoio: Bolsas no Brasil - Doutorado Direto