Analysis of Hepatitis C Virus Intrahost Diversity across the Coding Region by Ultradeep Pyrosequencing

Autor(es)

Lauck, Michael ^[1] ; Alvarado-Mora, Monica V. ^{[2, 3]} ; Becker, Ericka A. ^[1] ; Bhattacharya, Dipankar ^{[4, 5]} ; Striker, Rob ^{[4, 5, 6]} ; Hughes, Austin L. ^[7] ; Carrilho, Flair J. ^{[2, 3]} ; O'Connor, David H. ^[1] ; Rebello Pinho, Joao R. ^{[2, 3]}

Afiliação do(s) autor(es)

^[1] Univ Wisconsin, Dept Pathol & Lab Med, Madison, WI 53703 - USA

^[2] Univ Sao Paulo, Sch Med, Dept Gastroenterol, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Med, Sao Paulo Inst Trop Med, Lab Trop Gastroenterol & Hepatol, Sao Paulo - Brazil

^[4] Univ Wisconsin, Dept Med, Madison, WI - USA

^[5] Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 - USA

^[6] WS Middleton Mem Vet Assoc Hosp, Madison, WI - USA

^[7] Univ S Carolina, Dept Biol Sci, Columbia, SC 29208 - USA

• Tipo de documento: Artigo Científico
• Fonte: Journal of Virology; v. 86, n. 7, p. 3952-3960, APR 2012.
• Citações Web of Science: 38

Resumo

Hepatitis C virus (HCV) is the leading cause of liver disease worldwide. In this study, we analyzed four treatment-naive patients infected with subtype 1a and performed Roche/454 pyrosequencing across the coding region. We report the presence of low-level drug resistance mutations that would most likely have been missed using conventional sequencing methods. The approach described here is broadly applicable to studies of viral diversity and could help to improve the efficacy of direct-acting antiviral agents (DAA) in the treatment of HCV-infected patients. (AU)

• Processo FAPESP: 08/50461-6 - Estudo da variabilidade genotípica dos vírus da Hepatite B e Hepatite C na Colômbia
• Beneficiário: Monica Viviana Alvarado Mora
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 07/53457-7 - Estudo da variabilidade genotípica dos vírus das Hepatites B e C na Colômbia
• Beneficiário: João Renato Rebello Pinho
• Modalidade de apoio: Auxílio à Pesquisa - Regular