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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Analysis of Hepatitis C Virus Intrahost Diversity across the Coding Region by Ultradeep Pyrosequencing

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Lauck, Michael [1] ; Alvarado-Mora, Monica V. [2, 3] ; Becker, Ericka A. [1] ; Bhattacharya, Dipankar [4, 5] ; Striker, Rob [4, 5, 6] ; Hughes, Austin L. [7] ; Carrilho, Flair J. [3, 2] ; O'Connor, David H. [1] ; Rebello Pinho, Joao R. [2, 3]
Total Authors: 9
[1] Univ Wisconsin, Dept Pathol & Lab Med, Madison, WI 53703 - USA
[2] Univ Sao Paulo, Sch Med, Dept Gastroenterol, Sao Paulo - Brazil
[3] Univ Sao Paulo, Sch Med, Sao Paulo Inst Trop Med, Lab Trop Gastroenterol & Hepatol, Sao Paulo - Brazil
[4] Univ Wisconsin, Dept Med, Madison, WI - USA
[5] Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 - USA
[6] WS Middleton Mem Vet Assoc Hosp, Madison, WI - USA
[7] Univ S Carolina, Dept Biol Sci, Columbia, SC 29208 - USA
Total Affiliations: 7
Document type: Journal article
Source: Journal of Virology; v. 86, n. 7, p. 3952-3960, APR 2012.
Web of Science Citations: 38

Hepatitis C virus (HCV) is the leading cause of liver disease worldwide. In this study, we analyzed four treatment-naive patients infected with subtype 1a and performed Roche/454 pyrosequencing across the coding region. We report the presence of low-level drug resistance mutations that would most likely have been missed using conventional sequencing methods. The approach described here is broadly applicable to studies of viral diversity and could help to improve the efficacy of direct-acting antiviral agents (DAA) in the treatment of HCV-infected patients. (AU)