Associations between glutathione peroxidase-1 Pro198Leu polymorphism, selenium status, and DNA damage levels in obese women after consumption of Brazil nuts

Autor(es)

Cominetti, Cristiane ^[1] ; de Bortoli, Maritsa Carla ^[1] ; Purgatto, Eduardo ^[1] ; Ong, Thomas Prates ^[1] ; Moreno, Fernando Salvador ^[1] ; Garrido, Jr., Arthur Belarmino ^[2] ; Franciscato Cozzolino, Silvia Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Food & Expt Nutr, Fac Pharmaceut Sci, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med, Dept Gastroenterol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NUTRITION; v. 27, n. 9, p. 891-896, SEP 2011.
• Citações Web of Science: 33

Resumo

Objective: Alterations in selenium (Se) status may result in suboptimal amounts of selenoproteins, which have been associated with increased oxidative stress levels. The Pro198Leu polymorphism at the glutathione peroxidase-1 (GPx1) gene is supposed to be functional. The response of Se status, GPx activity, and levels of DNA damage to a Se supplementation trial between the genotypes related to that polymorphism was investigated. Methods: A randomized trial was conducted with 37 morbidly obese women. Participants consumed one Brazil nut, which provided approximately 290 mu g of Se a day, for 8 wk. Blood Se concentrations, erythrocyte GPx activity, and DNA damage levels were measured at baseline and at 8 wk. The results were compared by genotypes. Results: The genotype frequencies were 0.487, 0.378, and 0.135 for Pro/Pro (the wild-type genotype), Pro/Leu, and Leu/Leu, respectively. At baseline, 100% of the subjects were Se deficient, and after the supplementation, there was an improvement in plasma Se (P < 0.001 for Pro/Pro and Pro/Leu, P < 0.05 for Leu/Leu), erythrocyte Se (P = 0.00 for Pro/Pro and Pro/Leu, P < 0.05 for Leu/Leu), and GPx activity (P = 0.00 for Pro/Pro, P < 0.00001 for Pro/Leu, P < 0.001 for Leu/Leu). In addition, the Pro/Pro group showed a decrease in DNA damage after Brazil nut consumption compared with baseline (P < 0.005), and those levels were higher in Leu/Leu subjects compared with those with the wild-type genotype (P < 0.05). Conclusion: Consumption of one unit of Brazil nuts daily effectively increases Se status and increases GPx activity in obese women, regardless of GPx1 Pro198Leu polymorphism. However, the evaluated biomarkers showed distinct results in response to the supplementation when the polymorphism was considered. (c) 2011 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 07/50533-4 - Efeitos da suplementacao com castanha-do-brasil (bertholletia excelsa h.b.k.) sobre o estresse oxidativo em mulheres obesas e sua relacao com o polimorfismo pro 198leu no gene da glutationa peroxidade 1.
• Beneficiário: Silvia Maria Franciscato Cozzolino
• Modalidade de apoio: Auxílio à Pesquisa - Regular