Lower cytokine secretion ex vivo by natural killer T cells in HIV-infected individuals is associated with higher CD161 expression

Autor(es)

Snyder-Cappione, Jennifer E. ^[1] ; Loo, Christopher P. ^[1] ; Carvalho, Karina I. ^{[2, 3]} ; Kuylenstierna, Carlotta ^[4] ; Deeks, Steven G. ^[5] ; Hecht, Frederick M. ^[5] ; Rosenberg, Michael G. ^[6] ; Sandberg, Johan K. ^[4] ; Kallas, Esper G. ^{[2, 3]} ; Nixon, Douglas F. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Calif San Francisco, Div Expt Med, Dept Med, San Francisco, CA 94110 - USA

^[2] Univ Fed Sao Paulo, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sao Paulo - Brazil

^[4] Karolinska Univ Hosp, CIM, Dept Med, Karolinska Inst, Stockholm - Sweden

^[5] San Francisco Gen Hosp, Posit Hlth Program, San Francisco, CA 94110 - USA

^[6] Albert Einstein Coll Med, Jacobi Med Ctr, Bronx, NY 10467 - USA

• Tipo de documento: Artigo Científico
• Fonte: AIDS; v. 23, n. 15, p. 1965-1970, SEP 24 2009.
• Citações Web of Science: 28

Resumo

Objective: Natural killer T (NKT) cells are efficiently targeted by HIV and severely reduced in numbers in the circulation of infected individuals. The functional capacity of the remaining NKT cells in HIV-infected individuals is poorly characterized. This study measured NKT cell cytokine production directly ex vivo and compared these responses with both the disease status and NKT subset distribution of individual patients. Methods: NKT cell frequencies, subsets, and ex-vivo effector functions were measured in the peripheral blood mononuclear cells of HIV-infected patients and healthy controls by flow cytometry. We measured cytokines from NKT cells after stimulation with either a-galactosyl ceramide-loaded CD1d dimers (DimerX-alpha GalCer) or phorbol myristate acetate and ionomycin. Results: The frequencies of NKT cells secreting interferon-gamma and tumor necrosis factor-alpha were significantly lower in HIV-infected patients than healthy controls after DimerX-alpha GalCer treatment, but responses were similar after treatment with phorbol myristate acetate and ionomycin. The magnitude of the interferon-gamma response to DimerX-alpha GalCer correlated inversely with the number of years of infection. Both interferon-gamma and tumor necrosis factor-alpha production in response to DimerX-alpha GalCer correlated inversely with CD161 expression. Conclusion: The ex-vivo Th1 responses of circulating NKT cells to CD1d-glycolipid complexes are impaired in HIV-infected patients. NKT cell functions may be progressively lost over time in HIV infection, and CD161 is implicated in the regulation of NKT cell responsiveness. (C) 2009 Wolters Kluwer Health vertical bar Lippincott Williams \& Wilkins (AU)

• Processo FAPESP: 04/15856-9 - Análise prospectiva das características virológicas e imunológicas em indivíduos com infecção recente pelo HIV-1 das cidades de São Paulo e Santos, SP
• Beneficiário: Ricardo Sobhie Diaz
• Modalidade de apoio: Auxílio à Pesquisa - Temático