Biological effects of insulin on murine melanoma cells and fish erythrophoroma cells: a comparative study

Autor(es)

Luchs, Adriana ^[1] ; Sumida, Doris Hissako ^[2] ; Visconti, Maria Aparecida ^[3] ; Castrucci, Ana Maria de Lauro ^[4]

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo (USP). Instituto de Ciências Biomédicas - Brasil

^[2] Universidade de São Paulo (USP). Instituto de Biociências - Brasil

^[3] Universidade de São Paulo (USP). Instituto de Biociências - Brasil

^[4] Universidade de São Paulo (USP). Instituto de Biociências. - Brasil

• Tipo de documento: Artigo Científico
• Fonte: General and Comparative Endocrinology; v. 156, n. 2, p. 218-223, Apr. 2008.
• Área do conhecimento: Ciências Biológicas - Fisiologia
• Assunto(s): Melanócitos Insulina

Resumo

Insulin is the hormone that plays an essential role in metabolism and mitosis of normal and tumor cells, exerting its pleiotropic effects through binding to specific membrane receptors and promoting the phosphorylation of tyrosine residues of the receptor itself and of other components of the signaling pathway. The aim of this study was to investigate the effects of insulin on melanogenesis and cell growth in three different cell lines: the goldfish GEM-81 erythrophoroma cells (undifferentiated and differentiated with 1.5% dimethylsulfoxide-DMSO), and the murine B16F10 and Cloudman S91 melanoma cells. Undifferentiated GEM-81 and B16F10 cells responded to insulin with a small increase of cell proliferation, whereas S91 cells responded with a decrease of growth. In the two mammalian cell lines, and in DMSO-differentiated GEM-81 cells, the hormone strongly inhibited melanogenesis, by decreasing tyrosinase activity. In undifferentiated GEM-81 cells, insulin had no effect on tyrosinase activity. An increase in the tyrosine phosphorylation status of pp185 (insulin receptor substrate 1 and 2-IRS-1/2) phosphorylation degree was observed in S91 mouse melanoma and in differentiated GEM-81 erythrophoroma cells, suggesting that this specific protein was maintained during transformation process and participates in insulin signaling. Our results imply an ancient and diverse history of the insulin signaling system in vertebrate pigment cells. (AU)

• Processo FAPESP: 97/06896-1 - Aquisição de microfisômetro (cytosensor - molecular devices) para determinar atividade funcional de células em cultura e tiras de tecido em tempo real
• Beneficiário: Regina Pekelmann Markus
• Modalidade de apoio: Auxílio à Pesquisa - Programa Infraestrutura - Equipamentos
• Processo FAPESP: 01/10469-9 - Modulacao da melanopsina pelos hormonios ( melatonina, endotelina e prolactina) em celulas pigmentares de peixes, anfibios e mamiferos.
• Beneficiário: Doris Hissako Matsushita
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 00/08785-7 - Receptores e vias de sinalização de insulina em células de melanoma murino S-91 de cloudman
• Beneficiário: Adriana Luchs
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 99/00131-9 - Regulacao hormonal e mecanismos de transducao de sinal em celulas pigmentares de teleosteos, normais e transformadas.
• Beneficiário: Maria Aparecida Visconti
• Modalidade de apoio: Auxílio à Pesquisa - Regular