A decrease in retinal progenitor cells is associated with early features of diabetic retinopathy in a model that combines diabetes and hypertension

Autor(es)

Mendonca, Jacqueline ^[1] ; de Faria, Lopes ^[1] ; Silva, Kamila Cristina ^[1] ; Boer, Patricia Aline ^[1] ; Cavalcanti, Tiago Correa ^[1] ; Brunini Rosales, Mariana Aparecida ^[1] ; Ferrari, Ana Luiza ^[1] ; Lopes de Faria, Jose Butori ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Med Sci, Dept Internal Med, Renal Pathophysiol Lab Invest Diabet Complicat, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR VISION; v. 14, n. 199, p. 1680-1691, SEP 11 2008.
• Citações Web of Science: 7

Resumo

Purpose: Hyperglycemia and hypertension contribute to the development of diabetic retinopathy, and this may involve alterations in the normal retinal cell cycle. In this work, we examined the influence of diabetes and hypertension on retinal cell replication in vivo and the relationship between these changes and several early markers of diabetic retinopathy. Methods: Diabetes was induced with streptozotocin in 4- and 12-week-old spontaneously hypertensive rats (SHR) and their Wistar Kyoto (WKY) controls. The rats were killed 15 days later. Retinal cells stained with bromodeoxyuridine (BrdU) were seen in rats of both ages. Results: In 12-week-old rats, the number of BrdU-positive retinal cells was higher in SHR than in WKY rats. After 15 days of diabetes mellitus, there was a marked reduction in cell replication only in diabetic SHR (p=0.007). The BrdU-positive cells expressed neural, glial, or vascular progenitor markers. There was greater expression of p27(Kip1) in the ganglion cell layer of both diabetic groups (p=0.05), whereas in the inner nuclear layer there was enhanced expression only in diabetic SHR (p=0.02). There was a marked increase in the retinal expression of fibronectin (p=0.04) and vascular endothelial growth factor (p=0.02) in diabetic SHR that was accompanied by blood-retinal barrier breakdown (p=0.01). Discussion: Concomitant diabetes and hypertension attenuated the proliferation of retinal cells, and it is associated with an increase in p27(Kip1) expression, fibronectin accumulation, and blood-retinal barrier breakdown. The replicative retinal cells displayed characteristics of progenitor cells. (AU)

• Processo FAPESP: 05/58189-5 - A hipertensão arterial acentua a neurodegeneração retiniana na patogênese da retinopatia diabética? Possível papel da hipertensão na exacerbação da RD
• Beneficiário: Jacqueline Mendonça Lopes de Faria
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 04/00455-9 - A genetica da hipertensao arterial pode modificar a resposta inflamatoria e oxidativa na patogenese da retinopatia diabetica? possivel papel da hipertensao na exacerbacao da rd.
• Beneficiário: Jacqueline Mendonça Lopes de Faria
• Modalidade de apoio: Auxílio à Pesquisa - Regular