Characterization of the C-terminal half of human juvenile myoclonic epilepsy protein EFHC1: Dimer formation blocks Ca2+ and Mg2+ binding to its functional EF-hand

Autor(es)

Murai, Marcelo J. ^[1] ; Sassonia, Rogerio C. ^[2] ; Zamboni, Andre H. ^[1] ; Conte, Fabio F. ^[1] ; Martins-de-Souza, Daniel ^[3] ; Aparicio, Ricardo ^[2] ; de Oliveira, Marcelo G. ^[2] ; Lopes-Cendes, Iscia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Med Sci, Dept Med Genet, BR-13083970 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Inst Chem, BR-13083970 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Inst Biol, Dept Biochem, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Archives of Biochemistry and Biophysics; v. 477, n. 1, p. 131-138, SEP 1 2008.
• Citações Web of Science: 5

Resumo

Human EFHC1 is a member of the EF-hand superfamily of Ca2+-binding proteins with three DM10 domains of unclear function. Point mutations in the EFHC1 gene are related to juvenile myoclonic epilepsy, a fairly common idiopathic generalized epilepsy. Here, we report the first structural and thermodynamic analyses of the EFHC1C-terminus (residues 403-640; named EFHC1C), comprising the last DM10 domain and the EF-hand motif. Circular dichroism spectroscopy revealed that the secondary structure of EFHC1C is composed by 34% of alpha helices and 17% of beta -strands. Size exclusion chromatography and mass spectrometry showed that under oxidizing condition EFHC1C dimerizes through the formation of disulfide bond. Tandem mass spectrometry (MS/MS) analysis of peptides generated by trypsin digestion suggests that the Cys575 is involved in intermolecular S-S bond. In addition, DTNB assay showed that each reduced EFHC1C molecule has one accessible free thiol. Isothermal titration calorimetry (ITC) showed that while the interaction between Ca2+ and EFHC1C is enthalpically driven (Delta H= -58.6 to -67 kj/mol and T Delta S = -22.5 to -31 kj/mol) the interaction between Mg2+ and EFHC1 C involves an entropic gain,and is similar to 5 times less enthalpically favorable (Delta H= -11.7 to -14kj/mol and T Delta S=21.9 to 19kj/mol) than for Ca2+ binding. It was also found that under reducing condition Ca2+ or Mg2+ ions bind to EFH1C in a1/1 molar ratio, while under oxidizing condition this ratio is reduced, showing that EFH1C dimerization block Ca2+ and Mg2+ binding. (AU)

• Processo FAPESP: 05/03234-6 - Estudos estruturais da LGI1 humana
• Beneficiário: Ricardo Aparicio
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 04/03600-0 - Estudos funcionais do gene LGI1 no contexto da epilepsia de lobo temporal lateral
• Beneficiário: Iscia Teresinha Lopes Cendes
• Modalidade de apoio: Auxílio à Pesquisa - Regular