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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Coffee and Caffeine Protect against Liver Injury Induced by Thioacetamide in Male Wistar Rats

Texto completo
Autor(es):
Furtado, Kelly S. [1] ; Prado, Monize G. [2] ; Aguiar e Silva, Marco A. [2] ; Dias, Marcos C. [1] ; Rivelli, Diogo P. [3] ; Rodrigues, Maria A. M. [1] ; Barbisan, Luis F. [2, 1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] UNESP Sao Paulo State Univ, Sch Med, Dept Pathol, BR-18618970 Botucatu, SP - Brazil
[2] UNESP Sao Paulo State Univ, Inst Biosci, Dept Morphol, BR-18618970 Botucatu, SP - Brazil
[3] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin Chem & Toxicol, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY; v. 111, n. 5, p. 339-347, NOV 2012.
Citações Web of Science: 33
Resumo

Coffee intake has been inversely related to the incidence of liver diseases, although there are controversies on whether these beneficial effects on human health are because of caffeine or other specific components in this popular beverage. Thus, this study evaluated the protective effects of coffee or caffeine intake on liver injury induced by repeated thioacetamide (TAA) administration in male Wistar rats. Rats were randomized into five groups: one untreated group (G1) and four groups (G2G5) treated with the hepatotoxicant TAA (200 similar to mg/kg b.w., i.p.) twice a week for 8 similar to weeks. Concomitantly, rats received tap water (G1 and G2), conventional coffee (G3), decaffeinated coffee (G4) or 0.1% caffeine (G5). After 8 similar to weeks of treatment, rats were killed and blood and liver samples were collected. Conventional and decaffeinated coffee and caffeine intake significantly reduced serum levels of alanine aminotransferase (ALT) (p similar to<similar to 0.001) and oxidized glutathione (p similar to<similar to 0.05), fibrosis/inflammation scores (p similar to<similar to 0.001), collagen volume fraction (p similar to<similar to 0.01) and transforming growth factor beta-1 (TGF-beta 1) protein expression (p similar to=similar to 0.001) in the liver from TAA-treated groups. In addition, conventional coffee and caffeine intake significantly reduced proliferating cellular nuclear antigen (PCNA) S-phase indexes (p similar to<similar to 0.001), but only conventional coffee reduced cleaved caspase-3 indexes (p similar to<similar to 0.001), active metalloproteinase 2 (p similar to=similar to 0.004) and the number of glutathione S-transferase placental form (GST-P)-positive preneoplastic lesions (p similar to<similar to 0.05) in the liver from TAA-treated groups. In conclusion, conventional coffee and 0.1% caffeine intake presented better beneficial effects than decaffeinated coffee against liver injury induced by TAA in male Wistar rats. (AU)

Processo FAPESP: 07/54858-5 - Influência do consumo do café (com e sem cafeína) ou da cafeína isolada sobre a fibrose e a promoção da hepatocarcinogênese química em Ratos Wistar machos
Beneficiário:Kelly Silva Furtado
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 09/50890-7 - Influência do consumo do café (com e sem cafeína) ou da cafeína sobre a hepatotoxicidade induzida pela tiocetamida em Ratos Wistar machos
Beneficiário:Monize Gonçalves Do Prado
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 08/50985-5 - Avaliação da atividade moduladora do cafe/cafeina e da Ginkgo Biloba sobre o processo de carcinogênese química em diferentes modelos experimentais
Beneficiário:Luís Fernando Barbisan
Linha de fomento: Auxílio à Pesquisa - Regular