High expression of human monocyte iNOS mRNA induced by Paracoccidioides brasiliensis is not associated with increase in NO production

Autor(es)

Bordon-Graciani, Ana Paula ^[1] ; Dias-Melicio, Luciane Alarcao ^[2] ; Acorci-Valerio, Michele Janegitz ^[1] ; Araujo, Jr., Joao Pessoa ^[1] ; Victoriano de Campos Soares, Angela Maria ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Inst Biociencias, Dept Microbiol & Imunol, BR-18618970 Sao Paulo - Brazil

^[2] UNESP Univ Estadual Paulista, Fac Med, Dept Patol, BR-18618970 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Microbes and Infection; v. 14, n. 12, p. 1049-1053, OCT 2012.
• Citações Web of Science: 1

Resumo

In this study we investigated the role of nitric oxide (NO) in monocyte fungicidal activity against Paracoccidioides brasiliensis. We found that cells primed with IFN-gamma, TNF-alpha or GM-CSF and challenged with a high-(Pb18) or low-virulence (Pb265) strain of the fungus increase their fungicidal activity. Expression of iNOS mRNA was increased after priming cells with each cytokine, and tended to be inhibited by Pb18. Despite up-regulation of iNOS mRNA expression by Pb265, an equivalent increase in NO production was not detected, as metabolite levels were similar in all cultures. The results indicated that high expression of human monocyte iNOS mRNA induced by P brasiliensis is not correlated with NO concentrations produced. (C) 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. (AU)

• Processo FAPESP: 05/55570-0 - Efeito de prostaglandinas sobre os mecanismos efetores envolvidos na atividade fungicida de monocitos humanos ativados com diferentes citocinas e desafiados com cepas de alta e baixa virulencia do parac..
• Beneficiário: Angela Maria Victoriano de Campos Soares
• Modalidade de apoio: Auxílio à Pesquisa - Regular