Porphyromonas gingivalis infection at different gestation periods on fetus development and cytokines profile

Autor(es)

Michelin, M. C. A. N. ^[1] ; Teixeira, S. R. L. ; Ando-Suguimoto, E. S. ; Lucas, S. R. R. ^[1] ; Mayer, M. P. A. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dev Biol Lab, Dept Morphol & Genet, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Lab Oral Microbiol, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ORAL DISEASES; v. 18, n. 7, p. 648-654, OCT 2012.
• Citações Web of Science: 7

Resumo

Oral Diseases (2012) 18, 648654 Aim: Infective agents may affect pregnancy outcomes by deregulating homeostasis. Objectives: The effects of Porphyromonas gingivalis infection before and at different gestation periods were evaluated. Materials and Methods: Wistar rats infected via subcutaneous with P. gingivalis W83, one week before mating (BM), days 1 (PR1) and 11 of gestation (PR11), and controls were evaluated, and samples were obtained at the end of gestation. P. gingivalis was detected by PCR. Cytokine was determined by ELISA. Results: Infected rats had lower maternal gain of weight. Implantation was not observed in 2/12 BM rats. PR11 presented more fetal-placental resorptions and lower placenta/fetus weight than controls. P. gingivalis was detected in placenta and fetus. IL-6 and TNF-a levels were higher in placenta and serum of infected groups, except for TNF-a in placenta of PR1. IL-1 beta levels were higher in placenta of PR11, but lower in serum and placenta of PR1. There were no differences in IL-10 and PGE2 concentrations among the groups (P < 0.05). Conclusions: The experimental infection by P. gingivalis resulted in alterations in the gestational pattern and in fetal development. The consequences of infection at mid-gestation were more severe than at the beginning, possibly due to the induction of pro-inflammatory cytokines in the fetal compartment. (AU)

• Processo FAPESP: 07/57597-8 - Avaliacao dos efeitos causados na unidade feto-placentaria pela infeccao experimental por porphyromonas gingivalis em diferentes periodos gestacionais.
• Beneficiário: Marcia Pinto Alves Mayer
• Modalidade de apoio: Auxílio à Pesquisa - Regular