Co-nanoencapsulation of magnetic nanoparticles and selol for breast tumor treatment: in vitro evaluation of cytotoxicity and magnetohyperthermia efficacy

Autor(es)

Estevanato, Luciana L. C. ; Da Silva, Jaqueline R. ; Falqueiro, Andre M. ^[1] ; Mosiniewicz-Szablewska, Ewa ^[2] ; Suchocki, Piotr ^{[3, 4]} ; Tedesco, Antnio C. ^[1] ; Morais, Paulo C. ^[5] ; Lacava, Zulmira G. M. ^[6]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Lab Fotobiol & Fotomed, Dept Quim, Ribeirao Preto, SP - Brazil

^[2] Polish Acad Sci, Inst Phys, Warsaw - Poland

^[3] Warsaw Med Univ, Dept Drug Anal, Warsaw - Poland

^[4] Natl Med Inst, Dept Pharmaceut Chem, Warsaw - Poland

^[5] Univ Brasilia, Inst Fis, BR-70910900 Brasilia, DF - Brazil

^[6] Univ Brasilia, Inst Ciencias Biol, CNANO, BR-70910900 Brasilia, DF - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF NANOMEDICINE; v. 7, p. 5287-5299, 2012.
• Citações Web of Science: 17

Resumo

Antitumor activities have been described in selol, a hydrophobic mixture of molecules containing selenium in their structure, and also in maghemite magnetic nanoparticles (MNPs). Both selol and MNPs were co-encapsulated within poly(lactic-co-glycolic acid) (PLGA) nanocapsules for therapeutic purposes. The PLGA-nanocapsules loaded with MNPs and selol were labeled MSE-NC and characterized by transmission and scanning electron microscopy, electrophoretic mobility, photon correlation spectroscopy, presenting a monodisperse profile, and positive charge. The antitumor effect of MSE-NC was evaluated using normal (MCF-10A) and neoplastic (4T1 and MCF-7) breast cell lines. Nanocapsules containing only MNPs or selol were used as control. MTT assay showed that the cytotoxicity induced by MSE-NC was dose and time dependent. Normal cells were less affected than tumor cells. Cell death occurred mainly by apoptosis. Further exposure of MSE-NC treated neoplastic breast cells to an alternating magnetic field increased the antitumor effect of MSE-NC. It was concluded that selol-loaded magnetic PLGA-nanocapsules (MSE-NC) represent an effective magnetic material platform to promote magnetohyperthermia and thus a potential system for antitumor therapy. (AU)

• Processo FAPESP: 09/13208-3 - Preparação, caracterização e avaliação da atividade antitumoral in vitro de nanocápsulas contendo Selol.
• Beneficiário: André Miotello Falqueiro
• Modalidade de apoio: Bolsas no Brasil - Mestrado