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Effect of experimental photopolymerized coatings on the hydrophobicity of a denture base acrylic resin and on Candida albicans adhesion

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Autor(es):
Lazarin, Andrea Azevedo [1] ; Machado, Ana Lucia [1] ; Zamperini, Camila Andrade [1] ; Wady, Amanda Fucci [1] ; Palomari Spolidorio, Denise Madalena [2] ; Vergani, Carlos Eduardo [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] UNESP Univ Estadual Paulista, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, BR-14801903 Araraquara, SP - Brazil
[2] UNESP Univ Estadual Paulista, Araraquara Dent Sch, Dept Physiol & Pathol, BR-14801903 Araraquara, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: ARCHIVES OF ORAL BIOLOGY; v. 58, n. 1, p. 1-9, JAN 2013.
Citações Web of Science: 15
Resumo

Objective: This study investigated the effect of experimental photopolymerized coatings, containing zwitterionic or hydrophilic monomers, on the hydrophobicity of a denture base acrylic resin and on Candida albicans adhesion. Methods: Acrylic specimens were prepared with rough and smooth surfaces and were either left untreated (control) or coated with one of the following experimental coatings: 2-hydroxyethyl methacrylate (HE); 3-hydroxypropyl methacrylate (HP); and 2-trimethylammonium ethyl methacrylate chloride (T); and sulfobetaine methacrylate (S). The concentrations of these constituent monomers were 25%, 30% or 35%. Half of the specimens in each group (control and experimentals) were coated with saliva and the other half remained uncoated. The surface free energy of all specimens was measured, regardless of the experimental condition. C. albicans adhesion was evaluated for all specimens, both saliva conditioned and unconditioned. The adhesion test was performed by incubating specimens in C. albicans suspensions (1 x 10(7) cell/mL) at 37 degrees C for 90 min. The number of adhered yeasts were evaluated by XTT (2,3-bis{[}2-methoxy-4-nitro-5-sulfopheny1]-5-[phenylamino]carbonyl]-2 H-tetrazolium-hydroxide) method. Results: For rough surfaces, coatings S (30 or 35%) and HP (30%) resulted in lower absorbance values compared to control. These coatings exhibited more hydrophilic surfaces than the control group. Roughness increased the adhesion only in the control group, and saliva did not influence the adhesion. The photoelectron spectroscopy analysis (XPS) confirmed the chemical changes of the experimental specimens, particularly for HP and S coatings. Conclusions: S and HP coatings reduced significantly the adhesion of C. albicans to the acrylic resin and could be considered as a potential preventive treatment for denture stomatitis. (C) 2012 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 06/06842-0 - Efetividade de vernizes fotopolimerizáveis experimentais na redução da hidrofobicidade e adesão de Candida albicans em uma resina para base de próteses
Beneficiário:Ana Lucia Machado
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 06/00435-3 - Efetividade de vernizes fotopolimerizáveis experimentais na redução da hidrofobicidade e adesão de Candida albicans em uma resina para base de prótese
Beneficiário:Andréa Azevedo Lazarin
Modalidade de apoio: Bolsas no Brasil - Doutorado