Heterogeneous time-dependent response of adipose tissue during the development of cancer cachexia

Autor(es)

Batista, Jr., M. L. ^{[1, 2]} ; Neves, R. X. ^{[1, 2]} ; Peres, S. B. ^{[3, 4]} ; Yamashita, A. S. ^[1] ; Shida, C. S. ^[5] ; Farmer, S. R. ^[4] ; Seelaender, M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Canc Metab Res Grp, Sao Paulo - Brazil

^[2] Univ Mogi Das Cruzes, Ctr Integrated Biotechnol, Lab Adipose Tissue Biol, BR-08780911 Sao Paulo - Brazil

^[3] Univ Estadual Maringa, Dept Physiol Sci, Maringa, Parana - Brazil

^[4] Boston Univ, Sch Med, Dept Biochem, Boston, MA 02118 - USA

^[5] Univ Fed Sao Paulo, Inst Sci & Technol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Endocrinology; v. 215, n. 3, p. 363-373, DEC 2012.
• Citações Web of Science: 28

Resumo

Cancer cachexia induces loss of fat mass that accounts for a large part of the dramatic weight loss observed both in humans and in animal models; however, the literature does not provide consistent information regarding the set point of weight loss and how the different visceral adipose tissue depots contribute to this symptom. To evaluate that, 8-week-old male Wistar rats were subcutaneously inoculated with 1 ml (2 x 10(7)) of tumour cells (Walker 256). Samples of different visceral white adipose tissue (WAT) depots were collected at days 0, 4, 7 and 14 and stored at -80 degrees C (seven to ten animals/each day per group). Mesenteric and retroperitoneal depot mass was decreased to the greatest extent on day 14 compared with day 0. Gene and protein expression of PPAR gamma(2) (PPARG) fell significantly following tumour implantation in all three adipose tissue depots while C/EBP alpha (CEBPA) and SREBP-1c (SREBF1) expression decreased over time only in epididymal and retroperitoneal depots. Decreased adipogenic gene expression and morphological disruption of visceral WAT are further supported by the dramatic reduction in mRNA and protein levels of perilipin. Classical markers of inflammation and macrophage infiltration (f4/80, CD68 and MIF-1 alpha) in WAT were significantly increased in the later stage of cachexia (although showing a incremental pattern along the course of cachexia) and presented a depot-specific regulation. These results indicate that impairment in the lipid-storing function of adipose tissue occurs at different times and that the mesenteric adipose tissue is more resistant to the `fat-reducing effect' than the other visceral depots during cancer cachexia progression. Journal of Endocrinology (2012) 215, 363-373 (AU)

• Processo FAPESP: 10/51078-1 - Bases moleculares da caquexia: adipogênese e remodelagem da matriz extracelular do tecido adiposo branco de pacientes com câncer gastrointestinal
• Beneficiário: Miguel Luiz Batista Junior
• Linha de fomento: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 07/52782-1 - Bases moleculares da caquexia associada ao câncer: relação do tecido adiposo branco e macrófagos infiltrados
• Beneficiário: Miguel Luiz Batista Junior
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado