A potential role for an extracellular methanol oxidase secreted by Moniliophthora perniciosa in Witches' broom disease in cacao

Autor(es):Mostrar menos -

de Oliveira, Bruno V. ^[1] ; Teixeira, Gleidson S. ^[1] ; Reis, Osvaldo ^[1] ; Barau, Joan G. ^[1] ; Teixeira, Paulo Jose P. L. ^[1] ; do Rio, Maria Carolina S. ^{[2, 1]} ; Domingues, Romenia R. ^[2] ; Meinhardt, Lyndel W. ^[3] ; Paes Leme, Adriana F. ^[2] ; Rincones, Johana ^[1] ; Pereira, Goncalo A. G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas UNICAMP, Inst Biol, Lab Genom & Expressao, Dept Genet Evolucao & Bioagentes, BR-13083970 Campinas, SP - Brazil

^[2] CNPEM, Lab Nacl Biociencias LNBio, BR-13083970 Campinas, SP - Brazil

^[3] USDA ARS, Sustainable Perennial Crops Lab, Beltsville, MD 20705 - USA

• Tipo de documento: Artigo Científico
• Fonte: Fungal Genetics and Biology; v. 49, n. 11, p. 922-932, NOV 2012.
• Citações Web of Science: 10

Resumo

The hemibiotrophic basidiomycete fungus Moniliophthora perniciosa, the causal agent of Witches' broom disease (WBD) in cacao, is able to grow on methanol as the sole carbon source. In plants, one of the main sources of methanol is the pectin present in the structure of cell walls. Pectin is composed of highly methylesterified chains of galacturonic acid. The hydrolysis between the methyl radicals and galacturonic acid in esterified pectin, mediated by a pectin methylesterase (PME), releases methanol, which may be decomposed by a methanol oxidase (MOX). The analysis of the M. pernciosa genome revealed putative max and pme genes. Real-time quantitative RT-PCR performed with RNA from mycelia grown in the presence of methanol or pectin as the sole carbon source and with RNA from infected cacao seedlings in different stages of the progression of WBD indicate that the two genes are coregulated, suggesting that the fungus may be metabolizing the methanol released from pectin. Moreover, immunolocalization of homogalacturonan, the main pectic domain that constitutes the primary cell wall matrix, shows a reduction in the level of pectin methyl esterification in infected cacao seedlings. Although MOX has been classically classified as a peroxisomal enzyme, M. perniciosa presents an extracellular methanol oxidase. Its activity was detected in the fungus culture supernatants, and mass spectrometry analysis indicated the presence of this enzyme in the fungus secretome. Because M. pernciosa possesses all genes classically related to methanol metabolism, we propose a peroxisome-independent model for the utilization of methanol by this fungus, which begins with the extracellular oxidation of methanol derived from the demethylation of pectin and finishes in the cytosol. (C) 2012 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 06/59843-3 - Histologia da interação planta patógeno em Theobroma cacao L. infectado por Crinipellis perniciosa (Stahel) Singer durante a doença vassoura de bruxa: aspectos estruturais, histoquímicos e imunocitoquímicos
• Beneficiário: Maria Carolina Scatolin Do Rio
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 07/51030-6 - Estudo do metabolismo peroxissomal no fungo Moniliophthora perniciosa e seu possível papel na vassoura-de-bruxa do cacaueiro
• Beneficiário: Bruno Vaz de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 09/50119-9 - Estudo integrado e comparativo de três doenças fúngicas do cacau: vassoura-de-bruxa, monilíase e mal do facão, visando à compreensão de mecanismos de patogenicidade para o desenvolvimento de estratégias de controle
• Beneficiário: Gonçalo Amarante Guimarães Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Temático