Distribution and molecular characterization of hepatitis C virus (HCV) genotypes in patients with chronic infection from Pernambuco State, Brazil

Autor(es)

Alvarado-Mora, Monica V. ^[1] ; Moura, Izolda M. ^[2] ; Botelho-Lima, Livia S. ; Azevedo, Raymundo S. ^[3] ; Lopes, Edmundo ^[4] ; Carrilho, Flair J. ; Pinho, Joao R. R.

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Depto Gastroenterol, Lab Trop Gastroenterol & Hepatol, Sch Med, Sao Paulo - Brazil

^[2] Fed Univ Pernambuco UFPE, Clin Hosp, Cent Lab, Recife, PE - Brazil

^[3] Univ Sao Paulo, Sch Med, Dept Pathol, Sao Paulo - Brazil

^[4] Fed Univ Pernambuco UFPE, Dept Clin Med, Recife, PE - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VIRUS RESEARCH; v. 169, n. 1, p. 8-12, OCT 2012.
• Citações Web of Science: 2

Resumo

Hepatitis C virus (HCV) is a public health problem throughout the world and 3% of the world population is infected with this virus. It is estimated that 3-4 millions individuals are being infected every year. It has been estimated that around 1.5% of Brazilian population is anti-HCV positive and the Northeast region showed the highest prevalence in Brazil. The aim of this study was to characterize HCV genotypes circulating in Pernambuco State (PE), Brazil, located in the Northeast region of the country. This study included 85 anti-HCV positive patients followed up between 2004 and 2011. For genotyping, a 380bp fragment of HCV RNA in the NS5B region was amplified by nested PCR. Phylogenetic analysis was conducted using Bayesian Markov chain Monte Carlo simulation (MCMC) using BEAST v.1.5.3. From 85 samples, 63 (74.1%) positive to NS5B fragment were successfully sequenced. Subtype 1b was the most prevalent in this population (42-66.7%), followed by 3a (16-25.4%), 1a (4-6.3%) and 2b (1-1.6%). Twelve (63.1%) and seven (36.9%) patients with HCV and schistosomiasis were infected with subtypes 1b and 3a, respectively. Brazil is a large country with many different population backgrounds; a large variation in the frequencies of HCV genotypes is predictable throughout its territory. This study reports HCV genotypes from Pernambuco State where subtype 1b was found to be the most prevalent. Phylogenetic analysis suggests the presence of the different HCV strains circulating within this population. (C) 2012 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 08/50461-6 - Estudo da variabilidade genotípica dos vírus da Hepatite B e Hepatite C na Colômbia
• Beneficiário: Monica Viviana Alvarado Mora
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 11/50562-0 - Caracterização das mutações associadas a evolução do carcinoma hepatocelular (CHC) em pacientes infectados pelo vírus da hepatite B e da hepatite C
• Beneficiário: João Renato Rebello Pinho
• Modalidade de apoio: Auxílio à Pesquisa - Regular