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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Distribution and molecular characterization of hepatitis C virus (HCV) genotypes in patients with chronic infection from Pernambuco State, Brazil

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Author(s):
Alvarado-Mora, Monica V. [1] ; Moura, Izolda M. [2] ; Botelho-Lima, Livia S. ; Azevedo, Raymundo S. [3] ; Lopes, Edmundo [4] ; Carrilho, Flair J. ; Pinho, Joao R. R.
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Fac Med, Depto Gastroenterol, Lab Trop Gastroenterol & Hepatol, Sch Med, Sao Paulo - Brazil
[2] Fed Univ Pernambuco UFPE, Clin Hosp, Cent Lab, Recife, PE - Brazil
[3] Univ Sao Paulo, Sch Med, Dept Pathol, Sao Paulo - Brazil
[4] Fed Univ Pernambuco UFPE, Dept Clin Med, Recife, PE - Brazil
Total Affiliations: 4
Document type: Journal article
Source: VIRUS RESEARCH; v. 169, n. 1, p. 8-12, OCT 2012.
Web of Science Citations: 2
Abstract

Hepatitis C virus (HCV) is a public health problem throughout the world and 3% of the world population is infected with this virus. It is estimated that 3-4 millions individuals are being infected every year. It has been estimated that around 1.5% of Brazilian population is anti-HCV positive and the Northeast region showed the highest prevalence in Brazil. The aim of this study was to characterize HCV genotypes circulating in Pernambuco State (PE), Brazil, located in the Northeast region of the country. This study included 85 anti-HCV positive patients followed up between 2004 and 2011. For genotyping, a 380bp fragment of HCV RNA in the NS5B region was amplified by nested PCR. Phylogenetic analysis was conducted using Bayesian Markov chain Monte Carlo simulation (MCMC) using BEAST v.1.5.3. From 85 samples, 63 (74.1%) positive to NS5B fragment were successfully sequenced. Subtype 1b was the most prevalent in this population (42-66.7%), followed by 3a (16-25.4%), 1a (4-6.3%) and 2b (1-1.6%). Twelve (63.1%) and seven (36.9%) patients with HCV and schistosomiasis were infected with subtypes 1b and 3a, respectively. Brazil is a large country with many different population backgrounds; a large variation in the frequencies of HCV genotypes is predictable throughout its territory. This study reports HCV genotypes from Pernambuco State where subtype 1b was found to be the most prevalent. Phylogenetic analysis suggests the presence of the different HCV strains circulating within this population. (C) 2012 Elsevier B.V. All rights reserved. (AU)