Hepatocellular carcinoma (HCC) is the most frequent primary tumor of the liver. The main risk factors include chronic viral infection by HBV and HCV, cirrhosis, alcoholic liver disease / non-alcoholic and hereditary genetic disorders such as Wilson disease and hemochromatosis. Such diseases are related to molecular events that induce hepatocellular carcinogenesis, since they can interfere with DNA synthesis. Polymorphisms in xenobiotic metabolizing genes, such as family members glutathione S-transferases (GSTs) may lead to increased activation of carcinogens or decreased ability to inactivate them may increase the risk for cancer. This study aims to analyze the C341T polymorphism of the GSTP1 gene in patients with cirrhosis and hepatocellular carcinoma and in individuals with no history of cancer (control group), to identify biomarkers of susceptibility to this cancer. In this proposal we will evaluate 150 patients with pathologic diagnosis of cirrhosis or hepatocellular carcinoma and blood samples from 500 healthy controls. Some variables also will be analyzed: age, hepatitis B virus, hepatitis C, etilism, cirrhosis, diabetes mellitus, BMI (body mass ondex) and level of alpha fetoprotein. polymorphisms genotyping will be performed by PCR-RFLP technique. The molecular findings and information about risk factors will be evaluated statistically. The results may help to clarify the role of genetic differences in susceptibility to the effects of carcinogens and identify biomarkers of susceptibility.
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