Genetic Polymorphisms Related to Via Vitamin D in Patients with cirrhosis with or without Hepatocellular Carcinoma

• Grant number: 15/04338-1
• Support type: Regular Research Grants
• Duration: March 01, 2016 - August 31, 2018
• Field of knowledge: Health Sciences - Medicine
• Principal researcher: Rita de Cássia Martins Alves da Silva
• Grantee: Rita de Cássia Martins Alves da Silva
• Home Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São José do Rio Preto , SP, Brazil
• Assoc. researchers: Dorotéia Rossi Silva Souza

Abstract

Introduction - Cirrhosis, a chronic liver disease, is characterized by diffuse fibrosis process that changing the lobular architecture, resultant of increased cell proliferation and DNA synthesis in the regenerating nodules. Admittedly, cirrhosis of any etiology, confers a higher risk of hepatoceular carcinoma (HCC), which is the third cause of cancer death worldwide. In this context, there is a vitamin D (VD), with emphasis on various types of cancer, including HCC, for their potential inhibitory power in angiogenesis. Thus, the elucidation of the molecular mechanisms involved in the synthesis of VD and transportation can contribute to new therapies, as well as early diagnosis and prognosis of liver diseases. Objective - To evaluate the association between VDR polymorphisms (rs2228570 - FokI, rs731236 - TaqI, rs7975232 - ApaI and rs1544410 - BsmI), DBP (rs4588 and rs7041) and CYP27B1 (rs10877012) with cirrhosis, in patients with or without HCC, besides demographic, clinical and survival parameters. Casuistic and Methods - We will study 150 patients with cirrhosis and 200 individuals without clinical and biochemical signs of the disease (controls). The respective polymorphisms will be genotyped by PCR (polymerase chain reaction) in real time using TaqMan assays. A questionnaire will be applied for registration of clinical and anthropometric profile, and lifestyle habits. The data will be statistically analyzed with significance level to <0.05-P value. (AU)