Genotoxic and mutagenic effects of erythrosine B, a xanthene food dye, on HepG2 cells

Autor(es)

Drumond Chequer, Farah Maria ^[1] ; Venancio, Vinicius de Paula ^[1] ; Bianchi, Maria de Lourdes Pires ^[1] ; Greggi Antunes, Lusania Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food and Chemical Toxicology; v. 50, n. 10, p. 3447-3451, OCT 2012.
• Citações Web of Science: 25

Resumo

Erythrosine (ErB) is a xanthene and an US Food and Drug Administration approved dye used in foods, drugs and cosmetics. Although its utilization is permitted, ErB is described as inhibitor of enzymes and protein-protein interactions and is toxic to pituitary and spermatogenesis processes. However, the genotoxicity and mutagenicity of ErB is inconclusive in the literature. This study aimed to analyze the genotoxicity of this dye using the alkaline comet assay and is the first investigation to evaluate ErB mutagenicity using the cytokinesis block micronucleus cytome (CBMN-Cyt) assay in HepG2 cells. These cells were chosen because they produce phase I and phase II enzymes that can mimic in vivo metabolism. The cells were treated with seven concentrations (0.1-70.0 mu g mL(-1)) of ErB, and the results showed genotoxicity at the two highest concentrations and mutagenicity at six concentrations. Furthermore, as micronuclei result from clastogenic and aneugenic processes, while comet assay is often considered more sensitive and detects DNA single strain breaks, we suggest that an aneugenic is responsible for the observed damage. Although ErB is approved for use in the food, cosmetic and pharmaceutical industries, it must be used carefully because it damages the DNA structure. (C) 2012 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 11/01755-0 - Avaliação toxicogenética dos corantes de alimentos eritrosina e quinolina amarela e seus subprodutos: análise eletroquímica e da expressão dos genes TP53 e COX-2 em células hepáticas
• Beneficiário: Farah Maria Drumond Chequer
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/14115-9 - Avaliação toxicogenética, análise eletroquímica e perfil da expressão de genes associados aos danos no DNA em células HepG2 expostas aos corantes para alimentos eritrosina, quinolina amarela e seus subprodutos
• Beneficiário: Lusânia Maria Greggi Antunes
• Modalidade de apoio: Auxílio à Pesquisa - Regular