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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

NMDA receptor blockade impairs the muscarinic conversion of sub-threshold transient depression into long-lasting LTD in the hippocampus-prefrontal cortex pathway in vivo: Correlation with gamma oscillations

Texto completo
Autor(es):
Lopes-Aguiar, Cleiton [1] ; Bueno-Junior, Lezio Soares [1] ; Ruggiero, Rafael Naime [1] ; Romcy-Pereira, Rodrigo Neves [1, 2] ; Leite, Joao Pereira [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Neurosci & Behav Sci, Ribeirao Preto, SP - Brazil
[2] Univ Fed Rio Grande Norte UFRN, Inst Brain, BR-59056450 Natal, RN - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Neuropharmacology; v. 65, p. 143-155, FEB 2013.
Citações Web of Science: 10
Resumo

Cholinergic fibers from the brainstem and basal forebrain innervate the medial prefrontal cortex (mPFC) modulating neuronal activity and synaptic plasticity responses to hippocampal inputs. Here, we investigated the muscarinic and glutamatergic modulation of long-term depression (LTD) in the intact projections from CA1 to mPFC in vivo. Cortical-evoked responses were recorded in urethane-anesthetized rats for 30 min during baseline and 4 h following LTD. In order to test the potentiating effects of pilocarpine (PILO), independent groups of rats received either a microinjection of PILO (40 nmol; i.c.v.) or vehicle, immediately before or 20 min after a sub-threshold LTD protocol (600 pulses, 1 Hz; LFS600). Other groups received either an infusion of the selective NMDA receptor antagonist (AP7; 10 nmol; intra-mPFC) or vehicle, 10 min prior to PILO preceding LFS600, or prior to a supra-threshold LTD protocol (900 pulses, 1 Hz; LFS900). Our results show that PILO converts a transient cortical depression induced by LFS600 into a robust LTD, stable for at least 4 h. When applied after LFS600, PILO does not change either mPFC basal neurotransmission or late LTD. Our data also indicate that NMDA receptor pre-activation is essential to the muscarinic enhancement of mPFC synaptic depression, since AP7 microinjection into the mPFC blocked the conversion of transient depression into long-lasting LTD produced by PILO. In addition, AP7 effectively blocked the long-lasting LTD induced by LFS900. Therefore, our findings suggest that the glutamatergic co-activation of prefrontal neurons is important for the effects of PILO on mPFC synaptic depression, which could play an important role in the control of executive and emotional functions. (C) 2012 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 07/57484-9 - Modulação colinérgica da plasticidade sináptica cortical: controle muscarínico da depressão de longa duração (Ltd) na via hipocampo córtex pré frontal de ratos in vivo
Beneficiário:Cleiton Lopes Aguiar
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 05/56447-7 - Pesquisa através de imagens por ressonância magnética de alto campo destinadas a estudos em humanos
Beneficiário:João Pereira Leite
Linha de fomento: Auxílio à Pesquisa - Programa CINAPCE - Temático
Processo FAPESP: 09/54410-0 - Papel da plasticidade sináptica na via hipocampo - córtex pré-frontal medial de ratos em livre-movimento submetidos a modelos de psicose: implicações para o estudo de comorbidades psiquiátricas em modelos experimentais de epilepsia do lobo temporal
Beneficiário:Cleiton Lopes Aguiar
Linha de fomento: Bolsas no Brasil - Doutorado