Trypanosoma cruzi heparin-binding proteins present a flagellar membrane localization and serine proteinase activity

Autor(es)

Oliveira-, Jr., F. O. R. ^[1] ; Alves, C. R. ^[2] ; Silva, F. S. ^[2] ; Cortes, L. M. C. ^[2] ; Toma, L. ^[3] ; Boucas, R. I. ^[3] ; Aguilar, T. ^[3] ; Nader, H. B. ^[3] ; Pereira, M. C. S. ^[1]

Afiliação do(s) autor(es)

^[1] Fiocruz MS, Inst Oswaldo Cruz, Lab Ultraestrutura Celular, BR-21045900 Rio De Janeiro, RJ - Brazil

^[2] Fiocruz MS, Inst Oswaldo Cruz, Lab Biol Mol & Doencas Endemicas, BR-21045900 Rio De Janeiro, RJ - Brazil

^[3] Univ Fed Sao Paulo, UNIFESP, Dept Bioquim, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Parasitology; v. 140, n. 2, p. 171-180, FEB 2013.
• Citações Web of Science: 3

Resumo

Heparin-binding proteins (HBPs) play a key role in Trypanosoma cruzi-host cell interactions. HBPs recognize heparan sulfate (HS) at the host cell surface and are able to induce the cytoadherence and invasion of this parasite. Herein, we analysed the biochemical properties of the HBPs and also evaluated the expression and subcellular localization of HBPs in T. cruzi trypomastigotes. A flow cytometry analysis revealed that HBPs are highly expressed at the surface of trypomastigotes, and their peculiar localization mainly at the flagellar membrane, which is known as an important signalling domain, may enhance their binding to HS and elicit the parasite invasion. The plasmon surface resonance results demonstrated the stability of HBPs and their affinity to HS and heparin. Additionally, gelatinolytic activities of 70 kDa, 65.8 kDa and 59 kDa HBPs over a broad pH range (5.5-8.0) were revealed using a zymography assay. These proteolytic activities were sensitive to serine proteinase inhibitors, such as aprotinin and phenylmethylsulfonyl fluoride, suggesting that HBPs have the properties of trypsin-like proteinases. (AU)

• Processo FAPESP: 07/59801-1 - Estudo do envolvimento dos proteoglicanos na interacao entre celulas tumorais e estromais na formacao do tumor coloretal e de prostata
• Beneficiário: Leny Toma
• Modalidade de apoio: Auxílio à Pesquisa - Regular