Long-term amphetamine treatment exacerbates inflammatory lung reaction while decreases airway hyper-responsiveness after allergic stimulus in rats

Autor(es):Mostrar menos -

Ligeiro de Oliveira, Ana Paula ^[1] ; Lino-dos-Santos-Franco, Adriana ^[2] ; Acceturi, Beatriz Golega ^[2] ; Hamasato, Eduardo Kenji ^[3] ; Machado, Isabel Daufenback ^[4] ; Gimenes Junior, Joao Antonio ^[2] ; Vieira, Rodolfo de Paula ^[1] ; Damazo, Amilcar Sabino ^[5] ; Poliselli Farsky, Sandra Helena ^[4] ; Tavares-de-Lima, Wothan ^[2] ; Palermo-Neto, Joao ^[3]

Afiliação do(s) autor(es)

^[1] Nove de Julho Univ, Post Grad Program Biophoton Appl Hlth Sci, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Pharmacol, BR-05508900 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Vet Med, Lab Appl Pharmacol & Toxicol, BR-05508900 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Dept Clin & Toxicol Anal, Fac Pharmaceut Sci, BR-05508900 Sao Paulo - Brazil

^[5] Fed Univ Cuiaba, Dept Basic Sci Hlth, Fac Med Sci, Cuiaba, MT - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Immunopharmacology; v. 14, n. 4, p. 523-529, DEC 2012.
• Citações Web of Science: 7

Resumo

Asthma is an allergic lung disease can be modulated by drugs that modify the activity of central nervous system (CNS) such as amphetamine (AMPH). AMPH is a highly abused drug that exerts potent effects on behavior and immunity. In this study we investigated the mechanism involved in the effects of long-term AMPH treatment on the increased magnitude of allergic lung response. We evaluated mast cells degranulation, cytokines release, airways responsiveness and, expression of adhesion molecules. Male Wistar rats were treated with AMPH or vehicle (PBS) for 21 days and sensitized with ovalbumin (OVA) one week after the first injection of vehicle or AMPH. Fourteen days after the sensitization, the rats were challenged with an OVA aerosol, and 24 h later their parameters were analyzed. In allergic rats, the treatment with AMPH exacerbated the lung cell recruitment due increased expression of ICAM-1, PECAM-1 and Mac-1 in granulocytes and macrophages recovered from bronchoalveolar lavage. Elevated levels of IL-4, but decreased levels of IL-10 were also found in samples of lung explants after AMPH treatment. Conversely, the ex-vivo tracheal hyper-responsiveness to methacholine (MCh) was reduced by AMPH treatment, whereas the force contraction of tracheal segments due to in vitro antigen challenge remained unaltered. Our findings suggest that lung inflammation and airway hyper-responsiveness due to OVA challenge are under the distinct control of AMPH during long-term treatment. Our data strongly indicate that AMPH positively modulates allergic lung inflammation via the increase of ICAM-1, PECAM-1, Mac-1 and IL-4. AMPH also abrogates the release of the anti-inflammatory cytokine IL-10. (c) 2012 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 08/50766-1 - Repercussao pulmonar e sistemica apos exposicao de ratos ao formaldeido: papel regulador das especies reativas derivadas do oxigenio e do nitrogenio.
• Beneficiário: Adriana Lino dos Santos Franco
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 09/07208-0 - Modulação da inflamação pulmonar alérgica por adjuvantes ou tolerância imunológica
• Beneficiário: Momtchilo Russo
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 09/51886-3 - Neuroimunomodulação: fármacos, estresse e citocinas nas relações entre os sistemas nervoso, endócrino e imune
• Beneficiário: João Palermo Neto
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 07/55631-4 - Estudo da participação dos hormônios sexuais femininos na asma experimental induzida em linhagens de camundongos geneticamente selecionados (AIRmax e AIRmin)
• Beneficiário: Wothan Tavares de Lima
• Modalidade de apoio: Auxílio à Pesquisa - Regular