Karyotype characterization of two populations of Vernonia geminata (Asteraceae, Vernonieae) using banding and FISH techniques

Autor(es)

Oliveira, V. M. ^[1] ; Mansanares, M. E. ^[2] ; Semir, J. ^[1] ; Forni-Martins, E. R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Lab Biossistemat & Evolucao, Dept Biol Vegetal, Inst Biol, Campinas, SP - Brazil

^[2] Univ Fed Lavras, Setor Bot, Dept Biol, Lavras, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Genetics and Molecular Research; v. 11, n. 4, p. 4204-4212, 2012.
• Citações Web of Science: 1

Resumo

In order to extend our knowledge concerning karyotypes of the genus Vernonia, we applied various techniques of chromosome banding, including AgNOR and triple staining with the fluorochromes CMA/DA/DAPI (CDD), and of fluorescent in situ hybridization (FISH) for the 45S rDNA probe to specimens of two populations of Vernonia geminata collected from an open-pasture area, in southern Brazil. B chromosomes were observed in one of the populations. Both populations of V. geminata presented a pair of CMA(3)(+) terminal bands and one pair of chromosomes with terminal AgNOR banding. The FISH evidenced, in one population, two pairs of small sites of 45S rDNA; these being two small terminal sites and two centromeric sites. In the other population, there was only one pair of small terminal sites and two sites in two B chromosomes, one in each chromosome. There was coincidence of localization between CMA(+) and NOR bands with one of the small terminal sites of 45S rDNA of one chromosome of the normal complement, but not in B chromosomes. (AU)

• Processo FAPESP: 04/13165-9 - Caracterização cariotípica de espécies de Vernonia Schreb. (Asteraceae, Vernonieae) com técnicas de diferencial longitudinal de cromossomos (bandamento e hibridação de DNA in situ)
• Beneficiário: Eliana Regina Forni Martins
• Modalidade de apoio: Auxílio à Pesquisa - Regular